Germ line-specific and sexually dimorphic expression of a dead end gene homologue in turbot (Scophthalmus maximus)
Germ line-specific and sexually dimorphic expression of a dead end gene homologue in turbot (Scophthalmus maximus)
复制标题
大菱鲆（大菱鲆）死端基因同源物的种系特异性和性二态性表达。
DOI：
10.1016/j.theriogenology.2013.06.016
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发表时间：
2013-10-01
期刊：
影响因子：
2.8
通讯作者：
Li, J.
中科院分区：
文献类型：
--
作者：
Lin, F.;Zhao, C. Y.;Li, J.
生殖细胞对性腺发育和生育是不可缺少的。然而，由于缺乏生殖细胞标志物，海洋鱼类生殖细胞发育的生理机制尚不清楚。死角基因（dead end, dnd）是硬骨鱼原始生殖细胞（PGCs）迁移和发育的重要组成部分。本研究在大菱鲆（Scophthalmus maximus）中鉴定了一种dnd同源基因（Smdnd），并研究了其在胚胎发生和性腺发育过程中的表达模式。推断出的Smdnd氨基酸序列具有Dnd同源物的几个保守基序，并且与其他Dnd蛋白具有较高的同源性。系统发育分析显示，SmDnd与硬骨鱼近亲关系密切。逆转录聚合酶链反应（RT-PCR）和原位杂交显示Smdnd转录本可以在生殖细胞中检测到，包括推定的PGC和成年男性和女性生殖细胞。此外，通过实时PCR观察到Smdnd在性腺发育过程中有一个有趣的两性二态表达。雌性大比目鱼性成熟前Smdnd表达显著高于雄性（P < 0.05）。由于雄性在精原细胞增殖和减数分裂期间Smdnd的上调，这种差异逐渐减小。这些结果表明Smdnd可以作为大菱鲆生殖细胞的标记物。此外，Smdnd表达的时间和性别差异表明，该基因在两性性腺发育中可能起着不同的作用。(C) 2013爱思唯尔公司版权所有。
Germ cells are indispensable for gonadal development and fertility. However, the physiological mechanisms regulating germ cell development in marine fish are poorly understood due to a lack of germ cell markers. The dead end (dnd) gene is a vertebrate-specific component of germplasm crucial for primordial germ cells (PGCs) migration and development in teleosts. In this study, we identified a dnd homologue (Smdnd) in turbot (Scophthalmus maximus) and investigated its expression pattern during embryogenesis and gonadal development. The deduced amino acid sequence of Smdnd shared several conserved motifs of Dnd homologues as well as high identity to other Dnd proteins. Phylogenetic analysis revealed that the SmDnd was closely related to its teleost counterparts. Reverse transcription polymerase chain reaction (RT-PCR) and in situ hybridization revealed that Smdnd transcripts could be exclusively detected in germ cells, including presumptive PGC and adult male and female germ cells. In addition, an interesting sexually dimorphic expression of Smdnd during gonadal development was observed by real-time PCR. Female turbot showed greater (P < 0.05) Smdnd expression than male before sex maturation. This difference reduced gradually due to the upregulation of Smdnd in the male during the period corresponding to spermatogonia proliferation and meiosis. These results indicate that Smdnd can be used as a germ cell marker in turbot. In addition, the temporal and sex differences in Smdnd expression indicate that this gene may play different roles in gonadal development in both sexes. (C) 2013 Elsevier Inc. All rights reserved.