Quantification of the atrazine-degrading Pseudomonas sp strain ADP in aquifer sediment by quantitative competitive polymerase chain reaction
Quantification of the atrazine-degrading Pseudomonas sp strain ADP in aquifer sediment by quantitative competitive polymerase chain reaction
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DOI：
10.1111/j.1574-6941.2002.tb00983.x
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发表时间：
2002-09-01
影响因子：
4.2
通讯作者：
Aamand, J
中科院分区：
文献类型：
--
作者：
Clausen, GB;Larsen, L;Aamand, J
广泛使用的除草剂阿特拉津及其一些降解产物是欧洲和美国地下水中最常见的外源性物质之一。细菌假单胞菌属菌株ADP（P. ADP）在自传递质粒pADP-1上具有编码阿特拉津矿化的基因。在本研究中，这种能力的菌株矿化阿特拉津在含水层沉积物中的有氧条件下，在10 degreesC进行了研究。在两种生长条件下，ADP都能够在14天内矿化超过50%的2.8 μ M阿特拉津。在阿特拉津平板上进行降解物计数（作为菌落形成单位（CFU）），并通过定量竞争性聚合酶链反应（cPCR）测定atzA基因拷贝数。atZA基因编码催化菌株矿化阿特拉津的第一步的酶。atzA基因的定量比CFU计数产生更高的数量。高浓度的硝酸盐抑制阿特拉津矿化和琼脂平板上的可培养性，但atZA拷贝数在整个实验中保持稳定。结果表明，在需氧和厌氧条件下使用P. ADP进行生物强化的潜力，以及使用cPCR作为独立于可培养性的监测细菌的工具。(C)2002年由Elsevier Science B. V.代表欧洲微生物学会联合会出版。
The widely used herbicide atrazine and some of its degradation products are among the most commonly found xenobiotics in groundwater in Europe as well as in the USA. The bacterium Pseudomonas sp. strain ADP (P. ADP) possesses genes encoding atrazine mineralization on the self-transmissible plasmid pADP-1. In the present study, this ability of the strain to mineralize atrazine in aquifer sediment under both aerobic and denitrifying conditions at 10degreesC was studied. P. ADP was able to mineralize more than 50% of 2.8 muM atrazine within 14 days under both growth conditions. Counts of degraders as colony forming units (CFU) on atrazine plates and counts of atzA gene copies as determined by quantitative competitive polymerase chain reaction (cPCR) were performed. The atZA gene encodes the enzyme which catalyzes the first step of atrazine mineralization by the strain. Quantification of the atzA gene gave rise to higher numbers than did counts of CFU. High nitrate concentrations inhibited atrazine mineralization and culturability on agar plates, but atZA copy numbers remained stable throughout the experiment. The results show a potential for bioaugmentation using P. ADP at both aerobic and denitrifying conditions and the use of cPCR as a tool for monitoring the bacteria independent of culturability. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.