The circadian clock and darkness control natural competence in cyanobacteria
The circadian clock and darkness control natural competence in cyanobacteria
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DOI：
10.1038/s41467-020-15384-9
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发表时间：
2020-04-03
影响因子：
16.6
通讯作者：
Golden, Susan S.
中科院分区：
文献类型：
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作者：
Taton, Arnaud;Erikson, Christian;Golden, Susan S.
蓝藻细长聚球藻是研究昼夜节律的模式生物。它天生具有转化能力——也就是说，它从环境中吸收DNA，但潜在的机制尚不清楚。在这里，我们使用全基因组筛选来鉴定 S. elongatus 自然转化所需的基因，包括编码保守的 IV 型菌毛的基因、已知与其他细菌的能力相关的基因等。菌毛的生物发生每天发生在早晨，而当黑暗的开始与黄昏的昼夜节律高峰重合时，自然转化达到最大。因此，蓝藻的活性状态受到生物钟的调节，并且可以适应昼长的季节变化。
The cyanobacterium Synechococcus elongatus is a model organism for the study of circadian rhythms. It is naturally competent for transformation-that is, it takes up DNA from the environment, but the underlying mechanisms are unclear. Here, we use a genome-wide screen to identify genes required for natural transformation in S. elongatus, including genes encoding a conserved Type IV pilus, genes known to be associated with competence in other bacteria, and others. Pilus biogenesis occurs daily in the morning, while natural transformation is maximal when the onset of darkness coincides with the dusk circadian peak. Thus, the competence state in cyanobacteria is regulated by the circadian clock and can adapt to seasonal changes of day length.