Chromosomal analysis of Barrett's cells: demonstration of instability and detection of the metaplastic lineage involved
Chromosomal analysis of Barrett's cells: demonstration of instability and detection of the metaplastic lineage involved
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DOI：
10.1038/modpathol.3800787
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发表时间：
2007-07-01
期刊：
影响因子：
7.5
通讯作者：
Soares, Jorge
中科院分区：
文献类型：
--
作者：
Chaves, Paula;Crespo, Mateus;Soares, Jorge
Barrett食管内衬柱状和杯状细胞，具有胃和肠的特征。尽管杯状细胞与恶性肿瘤之间存在关联，但并未证明其他柱状细胞谱系与肿瘤无关。染色体异常被描述在邻近Barrett瘤的化生中，但不知道涉及哪种化生谱系。这项工作评估的频率和类型的染色体异常的巴雷特食管无肿瘤和进行鉴定的化生细胞携带染色体增益。收集Barrett食管活检并进行短期细胞培养和细胞遗传学分析。联合免疫荧光/荧光原位杂交的情况下，表现出染色体增益通过使用抗血清对肠（MUC 2）和胃（MUC 5AC和MUC 6）脱粘蛋白和染色体pericentromeric α卫星DNA探针的染色体参与。每例患者在200个非重叠核中的斑点数（0，1，2，42）进行评分。分别评估柱状细胞和杯状细胞。在40/60例（67%）中实现了短期细胞培养。40例中27例（68%）有克隆异常，10例（25%）有四倍体（4 n）克隆。在14/40例（35%）中检测到结构改变，在1 q21，15 q15和15 q22处具有复发性断点。16/40（40%）发生了数量变化（7号和18号三体以及Y丢失）。柱状细胞7号和18号染色体的获得率分别为9.8%和7.9%，明显高于杯状细胞0.7%和1.9%（P <0.05）。这些变化在表现出胃和肠功能的细胞中检测到，并且在没有脱粘蛋白产生的细胞中更常见。结论：（1）染色体不稳定性在Barrett食管中是一种常见的表现。(2)两个化生群体的承诺，染色体增益是更频繁的柱状非杯状细胞比杯状细胞。(3)两种化生表型，胃和肠，同样涉及。
Barrett's esophagus is lined by columnar and goblets cells with gastric and intestinal characteristics. Despite the association between goblet elements and malignancy, it was not demonstrated that other columnar cells lineages are not related to neoplasia. Chromosomal abnormalities were described in metaplasia adjacent to Barrett's neoplasia, but it is unknown which metaplastic lineages are involved. This work assessed the frequency and the type of chromosomal abnormalities in Barrett's esophagus without neoplasia and performed the identification of the metaplastic cells carrying chromosomal gains. Barrett's esophagus biopsies were collected and processed for short-term cell culture and cytogenetic analysis. Combined immunofluorescence/ fluorescence in situ hybridization was performed in cases exhibiting chromosomal gains by using antisera against intestinal (MUC2) and gastric (MUC5AC and MUC6) apomucins and chromosome pericentromeric alpha satellite DNA probes for the chromosomes involved. Each case was scored for the number of spots (0, 1, 2, 42) in 200 nonoverlapping nuclei. Columnar and goblet cells were separately assessed. Short-term cell cultures were achieved in 40/60 cases (67%). There were clonal abnormalities in 27/40 cases (68%) and tetraploid (4n) clones in 10/40 (25%). Structural alterations were detected in 14/40 (35%) with recurrent breakpoints at 1q21, 15q15 and 15q22. Numerical changes (trisomies 7 and 18 and loss of Y) occurred in 16/40 (40%). Gains of chromosomes 7 and 18 were more frequent in columnar than in goblet cells (9.8% vs 0.7% (P < 0.05)) and (7.9 vs 1.9% (P < 0.05)) respectively. These alterations were detected in cells exhibiting gastric as well as intestinal features and were more frequent in cells without apomucin production. Conclusions: (1) chromosomal instability is a common finding in Barrett's esophagus without neoplasia. (2) The two metaplastic populations are committed, chromosomal gains being more frequent in columnar nongoblet than in goblet cells. (3) The two metaplastic phenotypes, gastric and intestinal, are equally involved.