Distribution of Galanin mRNA Containing Cells and Galanin Receptor Binding Sites in Human and Rat Hypothalamus
Distribution of Galanin mRNA Containing Cells and Galanin Receptor Binding Sites in Human and Rat Hypothalamus
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人和大鼠下丘脑含甘丙肽mRNA的细胞和甘丙肽受体结合位点的分布
DOI：
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发表时间：
1990
影响因子：
3.4
通讯作者：
G. Mengod
中科院分区：
文献类型：
--
作者：
C. Bonnefond;J. Palacios;A. Probst;G. Mengod
采用原位杂交组织化学和受体放射自显影技术研究了雄性大鼠下丘脑和对照人脑死后下丘脑组织中含有甘丙肽mRNA的细胞和甘丙肽受体结合位点的分布。用32P标记的寡核苷酸探针进行杂交实验。杂交信号的特异性是通过多个探针、竞争分析和Northern blot分析确定的。在大鼠和人类下丘脑室旁核、视上核和弓形核中发现了高水平的杂交。人中间核和后皮层周围核散在细胞也含有甘丙氨酸mRNA。在大鼠背内侧核中也发现了甘丙肽mRNA。用125I标记的猪丙氨酸受体放射自显像研究了丙氨酸受体位点的分布。通过与不同神经肽的竞争来评估结合的特异性。虽然甘丙肽在纳摩尔浓度下阻断了结合，但其他被检测的神经肽在10 - 7 M浓度下无效。大鼠和人下丘脑视前区、腹内侧核和外侧核的丙氨酸结合位点密度最高。相反，在室旁核、视上核和弓形核中观察到极低密度的结合位点。我们的研究结果表明，在大鼠和人类下丘脑中，表达丙氨酸的神经元与丙氨酸受体的分布是互补的。这表明，丙氨酸受体并不位于丙氨酸能神经元的细胞体上，而可能位于这些细胞的突触前或突触后。
The distribution of cells containing galanin mRNA and that of galanin receptor binding sites were investigated using in situ hybridization histochemistry and receptor autoradiography in male rat hypothalamus and in postmortem hypothalamic tissues from control human brains. Oligonucleotide probes labelled with 32P were used for hybridization experiments. The specificity of the hybridization signal was ascertained using several probes, competition assays and Northern blot analysis. High levels of hybridization were found in the paraventricular, supraoptic and arcuate nuclei of rat and human hypothalamus. Human intermediate nuclei and scattered cells of the posterior perifornical nucleus also contained galanin mRNA. Galanin mRNA was also found in the dorsomedial nucleus of the rat. The distribution of galanin receptor sites was investigated by receptor autoradiography using 125I‐labelled porcine galanin. The specificity of the binding was assessed by competition with different neuropeptides. While galanin blocked the binding at nanomolar concentrations, the other neuropeptides examined were ineffective at 10‐7 M concentrations. The highest densities of galanin binding sites were seen in the preoptic area, ventromedial and lateral nuclei, of rat and human hypothalamus. In contrast, very low densities of binding sites were observed in the paraventricular, supraoptic and arcuate nuclei. Our results show that the distribution of neurons expressing galanin is complementary to that of galanin receptors in the rat and human hypothalamus. This suggests that receptors for galanin are not located on the cell bodies of galaninergic neurons, but are probably presynaptic on or postsynaptic to the processes of these cells.
大鼠中枢神经系统 125I-甘丙肽结合位点的放射自显影定量和解剖图谱。
影响因子：
2.8
作者：
Melander,T;Kohler,C;Nilsson,S;Hokfelt,T;Brodin,E;Theodorsson,E;Bartfai,T
通讯作者：
Bartfai,T