Effect of Dynamic Culture and Periodic Compression on Human Mesenchymal Stem Cell Proliferation and Chondrogenesis.
Effect of Dynamic Culture and Periodic Compression on Human Mesenchymal Stem Cell Proliferation and Chondrogenesis.
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DOI：
10.1007/s10439-015-1510-5
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发表时间：
2016-07
影响因子：
3.8
通讯作者：
Fisher JP
中科院分区：
文献类型：
--
作者：
Guo T;Yu L;Lim CG;Goodley AS;Xiao X;Placone JK;Ferlin KM;Nguyen BN;Hsieh AH;Fisher JP
我们最近开发了一种生物反应器，可以在动态培养中对工程组织施加剪切力和压缩力。在我们的系统中，藻酸盐水凝胶珠与封装的人间充质干细胞（hMSCs）培养在不同的动态条件下，同时进行周期性的，压缩力。开发了一种定制的压力传感器，用于跟踪施加剪切力和压缩力时的压力波动。与静态培养相比，动态培养可以在整个研究过程中保持较高的细胞群体。在仅施加切应力的情况下，qRT-PCR和免疫组化显示hMSC的软骨分化程度低于静态组。第二项研究表明，通过额外的机械压缩增强软骨形成分化。14天后，阿辛蓝染色显示压缩组形成更多的细胞外基质。通过qPCR证实了Sox 9、聚集蛋白聚糖和II型胶原等阳性软骨形成标志物的上调。我们的生物反应器提供了一种向工程软骨施加机械力的新方法。结果表明，动态培养与适当的机械刺激相结合，可以促进有效的祖细胞体外扩增，从而使临床相关的关节软骨细胞的治疗关节软骨缺损的文化。
We have recently developed a bioreactor that can apply both shear and compressive forces to engineered tissues in dynamic culture. In our system, alginate hydrogel beads with encapsulated human mesenchymal stem cells (hMSCs) were cultured under different dynamic conditions while subjected to periodic, compressive force. A customized pressure sensor was developed to track the pressure fluctuations when shear forces and compressive forces were applied. Compared to static culture, dynamic culture can maintain a higher cell population throughout the study. With the application of only shear stress, qRT-PCR and immunohistochemistry revealed that hMSCs experienced less chondrogenic differentiation than the static group. The second study showed that chondrogenic differentiation was enhanced by additional mechanical compression. After 14 days, alcian blue staining showed more extracellular matrix formed in the compression group. The upregulation of the positive chondrogenic markers such as Sox 9, aggrecan, and type II collagen were demonstrated by qPCR. Our bioreactor provides a novel approach to apply mechanical forces to engineered cartilage. Results suggest that a combination of dynamic culture with proper mechanical stimulation may promote efficient progenitor cell expansion in vitro, thereby allowing the culture of clinically relevant articular chondrocytes for the treatment of articular cartilage defects.