Polyelectrolyte mediated intra and intermolecular crosslinking in microgel-based etalons for sensing protein concentration in solution
Polyelectrolyte mediated intra and intermolecular crosslinking in microgel-based etalons for sensing protein concentration in solution
复制标题
DOI：
10.1039/c3cc00211j
复制
发表时间：
2013-01-01
影响因子：
4.9
通讯作者：
Serpe, Michael J.
中科院分区：
文献类型：
--
作者：
Islam, Molla R.;Serpe, Michael J.
生物素修饰的聚阳离子聚合物能够穿透聚（n -异丙基丙烯酰胺）-共丙烯酸微凝胶基标准子的金层。一旦穿透，聚阳离子与聚阴离子微凝胶交联，使其崩溃，导致反射光谱中光谱峰的蓝移。我们表明，蓝移的大小取决于暴露于标准子的生物素化多阳离子溶液的浓度。这种行为随后被用于生物传感应用。
Biotin modified polycationic polymers are capable of penetrating the Au overlayer of poly(N-isopropylacrylamide)-co-acrylic acid microgel-based etalons. Once penetrated, the polycations crosslink the polyanionic microgels, causing them to collapse, resulting in a concomitant blue shift of the spectral peaks in the reflectance spectrum. We show that the magnitude of the blue shift depends on the concentration of the biotinylated polycation solution exposed to the etalon. This behavior is subsequently used for biosensing applications.