New nonradioactive microspheres and more sensitive X-ray fluorescence to measure regional blood flow.
New nonradioactive microspheres and more sensitive X-ray fluorescence to measure regional blood flow.
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新的非放射性微球和更灵敏的 X 射线荧光来测量局部血流量。
DOI：
10.1152/ajpheart.1992.263.6.h1946
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Hoffman,JI
中科院分区：
文献类型：
--
作者：
Mori,H;Haruyama,S;Shinozaki,Y;Okino,H;Iida,A;Takanashi,R;Sakuma,I;Husseini,WK;Payne,BD;Hoffman,JI
我们开发了新的非放射性微球，并使用了比以前更灵敏的X射线荧光光谱仪来测量心脏和其他器官的局部血流。我们证明了8种重元素负载微球的化学稳定性，并通过比较急性和慢性犬实验中放射性和/或非放射性微球测量的重复流量来验证其用于区域血流测量的用途。波长色散光谱仪（Philips PW 1480）具有比先前描述的X射线荧光系统更高的灵敏度，并且减少了精确测量所需的微球数量。该系统的精细能量分辨率使得可以增加待定量的不同种类的微球的数量，但目前只有八种。我们还使用了同步辐射激发的能量色散光谱仪。单色同步辐射使我们能够获得比波长色散系统高得多的X射线荧光光谱的信号与背景比（对于Zr负载的微球多50 dB），并且将使得能够在比放射性方法更小的区域（< 20 mg）中分析荧光活性。
We developed new nonradioactive microspheres and used more sensitive X-ray fluorescence spectrometers than used previously to measure regional blood flow in the heart and other organs. We demonstrated the chemical stability of eight kinds of heavy element-loaded microspheres and validated their use for regional blood flow measurement by comparing duplicate flows measured with radioactive and/or nonradioactive microspheres in both acute and chronic dog experiments. The wavelength-dispersive spectrometer (Philips PW 1480) has a higher sensitivity than the previously described X-ray fluorescent system and reduced the number of microspheres required for accurate measurement. The fine energy resolution of this system makes it possible to increase the numbers of different kinds of microspheres to be quantitated, but at present only eight kinds are available. We also used a synchrotron radiation-excited energy dispersive spectrometer. The monochromatic synchrotron radiation allowed us to obtain much higher signal-to-background ratios of X-ray fluorescence spectra than with the wavelength-dispersive system (50 dB more for Zr-loaded microspheres) and will enable analysis of fluorescent activity in smaller regions (< 20 mg) than the radioactive method does.