CPEB, maskin, and cyclin B1 mRNA at the mitotic apparatus: Implications for local translational control of cell division
CPEB, maskin, and cyclin B1 mRNA at the mitotic apparatus: Implications for local translational control of cell division
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DOI：
10.1016/s0092-8674(00)00135-5
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发表时间：
2000-10-27
期刊：
影响因子：
64.5
通讯作者：
Richter, JD
中科院分区：
文献类型：
--
作者：
Groisman, I;Huang, YS;Richter, JD
在非洲爪蟾发育中，几种母体mRNA的表达受细胞质聚腺苷酸化的调控。CPEB和maskin是控制多聚腺苷酸化诱导翻译的两个因子，存在于动物胚胎极卵裂球的有丝分裂器上。细胞周期蛋白B1蛋白和mRNA，其翻译由多聚腺苷酸化调节，与CPEB和maskin共定位。CPEB与微管相互作用，并参与细胞周期蛋白B1 mRNA在有丝分裂器中的定位。在注射的胚胎中，破坏多聚腺苷酸化诱导的翻译的试剂抑制细胞分裂并促进纺锤体和中心体缺陷。其中两种药物抑制细胞周期蛋白B1蛋白的合成，一种药物对该过程几乎没有影响，破坏细胞周期蛋白B1 mRNA和蛋白的定位。这些数据表明，CPEB调节的mRNA翻译是重要的有丝分裂装置的完整性和细胞分裂。
In Xenopus development, the expression of several maternal mRNAs is regulated by cytoplasmic polyadenylation. CPEB and maskin, two factors that control polyadenylation-induced translation are present on the mitotic apparatus of animal pole blastomeres in embryos. Cyclin B1 protein and mRNA, whose translation is regulated by polyadenylation, are colocalized with CPEB and maskin. CPEB interacts with microtubules and is involved in the localization of cyclin B1 mRNA to the mitotic apparatus. Agents that disrupt polyadenylation-induced translation inhibit cell division and promote spindle and centrosome defects in injected embryos. Two of these agents inhibit the synthesis of cyclin B1 protein and one, which has little effect on this process, disrupts the localization of cyclin B1 mRNA and protein. These data suggest that CPEB-regulated mRNA translation is important for the integrity of the mitotic apparatus and for cell division.