The role of T3 surface molecules in the activation of human T cells: a two-stimulus requirement for IL 2 production reflects events occurring at a pre-translational level.
The role of T3 surface molecules in the activation of human T cells: a two-stimulus requirement for IL 2 production reflects events occurring at a pre-translational level.
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DOI：
10.4049/jimmunol.133.1.123
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发表时间：
1984-07
影响因子：
4.4
通讯作者：
A. Weiss;R. Wiskocil;J. Stobo
中科院分区：
文献类型：
--
作者：
A. Weiss;R. Wiskocil;J. Stobo
人T细胞白血病Jurkat被用作模型来检查T细胞活化的要求。这些研究表明，与T细胞特异性T3抗原反应的抗体不足以导致Jurkat细胞的活化，这由IL 2的分泌来确定。IL-2的产生只发生在第二个刺激，佛波酯PMA的存在。使用IL 2特异性cDNA探针，IL 2 RNA的出现，同样，只有当细胞用抗T3抗体和PMA刺激时才发生。这些结果证明了在人T细胞中基因表达的双刺激要求。
The human T cell leukemia Jurkat was used as a model to examine the requirements of T cell activation. These studies demonstrated that antibodies reactive with the T cell-specific T3 antigen were insufficient to result in the activation of Jurkat cells, determined by the secretion of IL 2. IL 2 production occurred only in the presence of a second stimulus, the phorbol ester PMA. With the use of an IL 2-specific cDNA probe, the appearance of IL 2 RNA, similarly, occurred only when cells were stimulated with both anti-T3 antibodies and PMA. These results demonstrate a two-stimulus requirement for gene expression in human T cells.