HORMONE-SENSITIVE LIPASE - SEQUENCE, EXPRESSION, AND CHROMOSOMAL LOCALIZATION TO 19 CENT-Q13.3
HORMONE-SENSITIVE LIPASE - SEQUENCE, EXPRESSION, AND CHROMOSOMAL LOCALIZATION TO 19 CENT-Q13.3
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DOI：
10.1126/science.3420405
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发表时间：
1988-09-16
期刊：
影响因子：
56.9
通讯作者：
SCHOTZ, MC
中科院分区：
文献类型：
--
作者：
HOLM, C;KIRCHGESSNER, TG;SCHOTZ, MC
激素敏感性脂肪酶是脂肪酸动员、整体能量稳态以及可能的类固醇生成中的关键酶，通过儿茶酚胺和胰岛素的可逆磷酸化受到严格控制。从克隆的大鼠脂肪细胞互补 DNA 预测的 757 个氨基酸序列与任何其他已知的脂肪酶或蛋白质没有同源性。活性控制磷酸化位点定位于明显亲水结构域中的 Ser563，并初步鉴定了脂质结合共有位点。一种或几种信使 RNA 种类（3.3、3.5 或 3.9 KB）在脂肪和类固醇生成组织以及心脏和骨骼肌中表达。人类激素敏感脂肪酶基因定位于染色体 19 cent-q13.3。
Hormone-sensitive lipase, a key enzyme in fatty acid mobilization, overall energy homeostasis, and possibly steroidogenesis,, is acutely controlled through reversible phosphorylation by catecholamines and insulin. The 757-amino acid sequence predicted from a cloned rat adipocyte complementary DNA showed no homology with any other known lipase or protein. The activity-controlling phosphorylation site was localized to Ser563 in a markedly hydrophlic domain, and a lipid-binding consensus site was tentatively identified. One or several messenger RNA species (3.3, 3.5, or 3.9 kilobases) were expressed in adipose and steroidogenic tissues and heart and skeletal muscle. The human hormone-sensitive lipase gene mapped to chromosome 19 cent-q13.3.