Halogenated hydrocarbon and hydroperoxide induced lipid peroxidation in rat tissue slices
Halogenated hydrocarbon and hydroperoxide induced lipid peroxidation in rat tissue slices
复制标题
卤代烃和氢过氧化物诱导大鼠组织切片中的脂质过氧化
DOI：
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
A. Tappel
中科院分区：
文献类型：
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作者：
M. Sano;A. Tappel
同时暴露于一种以上毒物对生物系统造成的损害可能比单个毒物造成的损害更大。在大鼠组织切片中测量硫代巴比妥酸反应物质（TBARS），以研究卤代烃（HHC）、过氧化叔丁基（BHP）或两种毒物的组合诱导的脂质过氧化。1 mM HHC孵育肝片产生TBARS的顺序为：BrCCL{sub 3} > CCl{sub 4} = C{sub 2}H{sub 2}Br{sub 2} > C{sub 2}H{sub 4}Cl{sub 2}；1 mM C{sub 2}H{sub 2}Br{sub 4}、C{sub 2}H{sub 2}Cl{sub 4}、CH{sub 2}Br{sub 2}、CH{sub 2}Cl{sub 2}或C{sub 2}HBrClF{sub 3}或0.05 mM BHP均未增加TBARS产量。与BHP孵育的肝片与BrCCl{sub 3}、CCl{sub 4}、C{sub 2}H{sub 4}Br{sub 2}、C{sub 2}H{sub 4}Cl{sub 2}、C{sub 2}H{sub 2}Br{sub 4}、C{sub 2}H{sub 2}Cl{sub 4}、C{sub 2}H{sub 2}Cl{sub 4}或C{sub 2}HBrClF{sub 3}协同产生TBARS，具体取决于使用的浓度。BHP和BrCCl{sub 3}在心脏切片中显示出协同作用，但在肾、睾丸、肺和脾切片中没有协同作用。HHC和BHP之间的协同作用取决于HHC、氧化剂浓度和个体组织。这种依赖性可能反映了这些化合物在个体组织中代谢的机制，以及影响脂质过氧化的其他组织变量。«少
Damage to biological systems exposed simultaneously to more that one toxicant may be amplified over damage produced by individual toxicants. Thiobarbituric acid reactive substances (TBARS) were measured in rat tissue slices to investigate lipid peroxidation induced by halogenated hydrocarbons (HHC), tert-butyl hydroperoxide (BHP), or a combination of the two types of toxicant. TBARS production by liver slices incubated with 1 mM HHC occurred in the following order: BrCCL{sub 3} > CCl{sub 4} = C{sub 2}H{sub 2}Br{sub 2} > C{sub 2}H{sub 4}Cl{sub 2}; 1 mM C{sub 2}H{sub 2}Br{sub 4}, C{sub 2}H{sub 2}Cl{sub 4}, CH{sub 2}Br{sub 2}, CH{sub 2}Cl{sub 2} or C{sub 2}HBrClF{sub 3} or 0.05 mM BHP did not increase TBARS production. TBARS production by liver slices incubated with BHP was synergistic in combination with BrCCl{sub 3}, CCl{sub 4}, C{sub 2}H{sub 4}Br{sub 2}, C{sub 2}H{sub 4}Cl{sub 2}, C{sub 2}H{sub 2}Br{sub 4}, C{sub 2}H{sub 2}Cl{sub 4}, or C{sub 2}HBrClF{sub 3}, depending upon concentrations used. A synergistic effect of BHP and BrCCl{sub 3} was shown in heart slices, but not in kidney, testes, lung, or spleen slices. Synergism between the HHC and BHP was dependent upon the HHC, oxidant concentrations, and the individual tissue. This dependency may reflect the mechanisms by which thesemore » compounds are metabolized in the individual tissues as well as other tissue variables that affect lipid peroxidation.« less