Characterization of the Largemouth Bass Virus in Cell Culture
Characterization of the Largemouth Bass Virus in Cell Culture
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DOI：
10.1577/1548-8667(1999)011
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发表时间：
1999-03
影响因子：
1.2
通讯作者：
Thea O. Piaskoski;J. Plumb;S. R. Roberts
中科院分区：
文献类型：
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作者：
Thea O. Piaskoski;J. Plumb;S. R. Roberts
摘要在细胞培养中，对大口黑鲈病毒（LMBV）进行了感染性、最适复制温度、乙醚敏感性和pH敏感性的研究。病毒诱导的细胞病变效应（CPE）在蓝鳃鱼苗-2（BF-2）和黑头呆鱼（FHM）细胞系中出现得最快，其中LMBV复制的最适温度为30°C。LMBV在BF-2细胞中呈一步生长曲线，24 h内达到108.6 TCID 50/mL（±0.12 log SD）。当用乙醚处理时，病毒显示出降低的感染性，但当在4°C下在pH 3-9的培养基中保持12小时时是稳定的。这些特征进一步支持了LMBV在虹彩病毒科中的分类。
Abstract The largemouth bass virus (LMBV) was characterized in cell culture, by infectivity in five cell lines of fish origin, optimum replication temperature, and ether and pH sensitivity. Viral induced cytopathic effect (CPE) appeared most rapidly in bluegill fry-2 (BF-2) and fathead minnow (FHM) cell lines where the optimum temperature for LMBV replication was 30°C. In a one-step growth curve in BF-2 cells, LMBV reached 108.6 TCID50/mL (±0.12 log SD) in 24 h. The virus showed reduced infectivity when treated with ether but was stable when held in medium with a pH 3–9 for 12 h at 4°C. These characteristics further support the classification of LMBV in the family Iridoviridae.