Surface-Enhanced Raman Spectroscopy of Bacterial Metabolites for Bacterial Growth Monitoring and Diagnosis of Viral Infection
Surface-Enhanced Raman Spectroscopy of Bacterial Metabolites for Bacterial Growth Monitoring and Diagnosis of Viral Infection
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细菌代谢物的表面增强拉曼光谱用于细菌生长监测和病毒感染诊断
DOI：
10.1021/acs.est.1c02552
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发表时间：
2021
影响因子：
11.4
通讯作者：
Vikesland, Peter J.
中科院分区：
文献类型：
--
作者：
Wang, Wei;Kang, Seju;Vikesland, Peter J.
细菌代谢产物是细菌代谢的中间产物，其产生反映了代谢活动。在这里，我们报告了使用表面增强拉曼光谱(SERS)来检测挥发性和非挥发性代谢物，并将该方法应用于细菌生长定量和病毒感染的诊断。对生长在琼脂平板上的细菌顶空中挥发性代谢物二甲基二硫化物随时间变化的SERS信号进行了检测和定量。此外，来自平板的SERS信号反映了营养物质的消耗和非挥发性代谢物的产生。代谢物积累量的测定可用于细菌的定量。在噬菌体病毒的存在下，细菌的新陈代谢受到抑制，SERS强度的相对降低反映了病毒的初始浓度。使用多变量分析，我们检测到病毒感染，预测准确率为93%。我们基于SERS的代谢物产生监测方法为病毒感染诊断提供了新的见解。
Bacterial metabolites are intermediate products of bacterial metabolism and their production reflects metabolic activity. Herein, we report the use of surface-enhanced Raman spectroscopy (SERS) for detection of both volatile and nonvolatile metabolites and the application of this approach for bacterial growth quantification and diagnosis of viral infection. The time-dependent SERS signal of the volatile metabolite dimethyl disulfide in the headspace above bacteria growing on an agar plate was detected and quantified. In addition, SERS signals arising from the plate reflected nutrient consumption and production of nonvolatile metabolites. The measurement of metabolite accumulation can be used for bacterial quantification. In the presence of bacteriophage virus, bacterial metabolism is suppressed, and the relative decrease in SERS intensity reflects the initial virus concentration. Using multivariate analysis, we detect viral infection with a prediction accuracy of 93%. Our SERS-based approach for metabolite production monitoring provides new insights toward viral infection diagnosis.