Role of phospholipase C and protein kinase C in Aspergillus nidulans during growth on pectin or glucose: Effects on germination and duplication cycle
Role of phospholipase C and protein kinase C in Aspergillus nidulans during growth on pectin or glucose: Effects on germination and duplication cycle
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DOI：
10.1007/s12223-010-0033-6
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发表时间：
2010-05-01
影响因子：
2.6
通讯作者：
Said, S.
中科院分区：
文献类型：
--
作者：
Chellegatti, M. A. S. C.;Yuvamoto, P. D.;Said, S.
PLC和PKC抑制剂对尼杜拉曲霉生长的影响与碳源有关。PLC抑制剂SPM和C48/80延迟了葡萄糖培养中的第一次核分裂，但在添加果胶的培养基中却促进了第一次核分裂。这些影响对转化PLC-A基因断裂(AP27)的突变体的影响较小。在以葡萄糖或果胶为底物的培养物中，新霉素也能延缓其萌发；然而，在葡萄糖或果胶上，细胞核分裂受到抑制，而在果胶上则受到刺激。这些影响在AP27中很小。Ro-31-8425和BIM(两种PKC抑制剂)对生长在葡萄糖或果胶上的细胞的作用也相反。在葡萄糖培养上，BIM延缓了发芽和第一次核分裂，而在果胶上，这两个参数都被刺激。在含有Ro-31-8425的葡萄糖或果胶上生长时，也检测到相反的效果。
The effects of PLC and Pkc inhibitors on Aspergillus nidulans depend on the carbon source. PLC inhibitors Spm and C48/80 delayed the first nuclear division in cultures growing on glucose, but stimulated it in media supplemented with pectin. Less intense were these effects on the mutant transformed with PLC-A gene rupture (AP27). Neomycin also delayed the germination in cultures growing on glucose or pectin; however, on glucose, the nuclear division was inhibited whereas in pectin it was stimulated. These effects were minor in AP27. The effects of Ro-31-8425 and BIM (both Pkc inhibitors) were also opposite for cultures growing on glucose or pectin. On glucose cultures of both strains BIM delayed germination and the first nuclear division, whereas on pectin both parameters were stimulated. Opposite effects were also detected when the cultures were growing on glucose or pectin in the presence of Ro-31-8425.