Airway and tissue mechanics in a murine model of asthma: alveolar capsule vs. forced oscillations
Airway and tissue mechanics in a murine model of asthma: alveolar capsule vs. forced oscillations
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DOI：
10.1152/japplphysiol.01129.2001
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发表时间：
2002-07-01
影响因子：
3.3
通讯作者：
Irvin, CG
中科院分区：
文献类型：
--
作者：
Tomioka, S;Bates, JHT;Irvin, CG
为了更好地解决支气管反应性炎症的功能后果，我们研究了两组BALB/c小鼠：非免疫对照组（n = 8）和免疫组和吸入卵白蛋白（n = 8）的挑战。肺泡囊（AC）测量气道阻力（Raw（AC））和肺弹性（EL）。强迫振荡（FO）技术独立地估计气道阻力（RawFO）和与组织弹性相关的参数H-ti。卵清蛋白免疫和激发小鼠支气管肺泡灌洗液中嗜酸性粒细胞的数量增加，对乙酰甲胆碱（MCh）的反应性增加。AC和FO技术的相应参数相关：RawAC与RawFO（r = 0.76）和EL与H-ti（r = 0.88，P < 0.0001）。AC和FO技术显示，响应于MCh的组织弹性显著增加，但气道阻力无显著增加。这些结果表明，AC和FO技术在小鼠中产生了基本相同的结果，即使当肺部不均匀时也是如此，并且小鼠对MCh和炎症的支气管收缩反应主要位于肺外周。
To better address the functional consequences of inflammation on bronchial responsiveness, we studied two groups of BALB/c mice: a nonimmunized control group (n = 8) and a group immunized and challenged with inhaled ovalbumin (n = 8). An alveolar capsule (AC) measured airway resistance (Raw(AC)) and lung elastance (EL). A forced oscillation (FO) technique independently estimated airway resistance (RawFO) and a parameter H-ti related to tissue elastance. Ovalbumin-immunized and -challenged mice had increased numbers of eosinophils in bronchoalveolar lavage and increased responsiveness to methacholine (MCh). Corresponding parameters from the AC and FO techniques were correlated: RawAC vs. RawFO (r = 0.76) and EL vs. H-ti (r = 0.88, P < 0.0001 in all cases). AC and FO techniques showed significant increases in tissue elastance in response to MCh but no significant increases in airway resistance. These results demonstrated that the AC and FO techniques yield essentially equivalent results in mice, even when the lung is inhomogeneous, and that the bronchoconstrictive responses to MCh and inflammation in mice are predominantly located in the lung periphery.