Studies on destruction of red blood cells. II. Chronic hemolytic anemia with paroxysmal nocturnal hemoglobinuria: Certain immunological aspects of the hemolytic mechanism with special reference to serum complement
Studies on destruction of red blood cells. II. Chronic hemolytic anemia with paroxysmal nocturnal hemoglobinuria: Certain immunological aspects of the hemolytic mechanism with special reference to serum complement
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DOI：
10.1172/jci101081
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发表时间：
1939-11-01
影响因子：
15.9
通讯作者：
Dingle, JH
中科院分区：
文献类型：
--
作者：
Ham, TH;Dingle, JH
在这种慢性溶血性贫血中，基本异常存在于红细胞中，当悬浮在酸化（pH值6 ~ 7）的血浆或血清中时，红细胞对溶血的敏感性增加，这些血浆或血清来自患者和相容血型的正常受试者。在相同条件下，患者血清不能溶血正常红细胞。溶血系统需要人体补体或类似补体的物质。溶血的机制可能是免疫学性质的，但不能确定患者红细胞的异常。当用于免疫兔子时，在病人的红细胞和正常受试者的红细胞之间没有观察到抗原差异。通过已知的解离抗原和抗体的方法未从患者细胞中除去溶血物质或抗体。患者红细胞对2种免疫型溶血素的敏感性增加，但对低渗溶血素的敏感性正常。盐，皂苷和钠牛磺胆酸盐
In this chronic hemolytic anemia the fundamental abnormality resides in the erythrocytes, which show increased susceptibility to hemolysis when suspended in acidified (pH 6 to 7) plasma or serum from patients and from normal subjects of compatible blood groups. The sera of patients did not hemolyze normal erythrocytes under the same conditions. Human complement, or a complement-like substance, was required by the hemolytic system. The mechanism of hemolysis may be immunological in nature, but the abnormality of the patient''s red blood cells is not denned. No antigenic difference, when used to immunize rabbits, was observed between the patient''s erythrocytes and those of a normal subject. No hemolytic substance or antibody was removed from the patient''s cells by procedures known to dissociate antigen and antibody. The patient''s erythrocytes showed increased susceptibility to 2 hemolysins of the immunological type, but a normal susceptibility to hemolysis by hypotonic solns. of NaCl, saponin, and Na taurocholate.