GalNAc pretreatment inhibits trapping of Bacillus thuringiensis Cry1Ac on the peritrophic membrane of Bombyx mori
GalNAc pretreatment inhibits trapping of Bacillus thuringiensis Cry1Ac on the peritrophic membrane of Bombyx mori
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DOI：
10.1016/j.febslet.2004.09.029
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发表时间：
2004-10-22
期刊：
影响因子：
3.5
通讯作者：
Hori, H
中科院分区：
文献类型：
--
作者：
Hayakawa, T;Shitomi, Y;Hori, H
家蚕 (Shunrei x Shogetsu) 对 Cry1Aa 敏感，对 Crv1Ac 具有抗性，这两种都是苏云金芽孢杆菌的杀虫蛋白。在初始观察期间，Cry1Aa 通过围营养膜 (PM) 的速度 (0.37 mug/mm(2) PM/h) 比 Cry1Ac (0.05 mug/mm(2) PM/h) 快得多。 Cry1Aa 和 Cry1Ac 均与 PM 结合，但只有 Cry1Ac 的结合被 N-乙酰半乳糖胺 (GalNAc) 特异性抑制。当 Cry1Ac 用 GalNAc 预处理时，Cry1Ac 渗透 PM 的速度要快得多。这些结果表明 Cry1Ac 通过糖侧链上的 GalNAc 结合 PM 蛋白。简要讨论了 PM 对家蚕 Cry1Ac 抗性的作用。 (C) 2004 年由 Elsevier B.V. 代表欧洲生化学会联合会出版。
Bombyx mori (Shunrei x Shogetsu) is sensitive to Cry1Aa and resistant to Crv1Ac, both insecticidal proteins of Bacillus thuringiensis. Cry1Aa passed through the peritrophic membrane (PM) much faster (0.37 mug/mm(2) PM/h) than Cry1Ac (0.05 mug/mm(2) PM/h) during the initial observation period. Both Cry1Aa and Cry1Ac bound to the PM but only the binding of Cry1Ac was specifically inhibited by N-acetylgalactosamine (GalNAc). When Cry1Ac was pretreated with GalNAc, Cry1Ac permeated the PM much faster. These results suggested that Cry1Ac bound a PM protein via GalNAc on a sugar side chain. The role of the PM on Cry1Ac resistance of B. mori was briefly discussed. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.