Analysis of transcriptome sequencing of sciatic nerves in Sprague-Dawley rats of different ages.
Analysis of transcriptome sequencing of sciatic nerves in Sprague-Dawley rats of different ages.
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DOI：
10.4103/1673-5374.241469
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发表时间：
2018-12
影响因子：
6.1
通讯作者：
Xu YB
中科院分区：
文献类型：
--
作者：
Liu JH;Tang Q;Liu XX;Qi J;Zeng RX;Zhu ZW;He B;Xu YB
衰老诱导的雪旺细胞活力下降可影响哺乳动物周围神经损伤后的再生。因此，有必要研究可能影响周围神经生物学功能的基因表达的年龄相关变化。选取10只1周龄和10只12月龄健康雄性sd大鼠，按年龄分为幼鼠组（1周龄）和成鼠组（12月龄）。采用下一代测序（NGS）和生物信息学方法比较幼龄和成年大鼠坐骨神经mRNA的表达（n = 4/组）。采用定量反转录聚合酶链反应（n = 6/组）检测18组差异表达mRNA （demmrna）。结果显示：(1)与幼龄大鼠相比，成年大鼠有3608组DEmRNAs。其中，上调基因组2684组，下调基因组924组。它们的功能主要涉及细胞活力、增殖、分化、再生和髓鞘形成。(2)在成年大鼠中，所有DEmRNAs增加最明显的基因是Thrsp (log2FC = 9.01, P < 0.05)，减少最明显的基因是Col2a1 （log2FC = -8.89, P < 0.05）。(3)基因本体论分析显示，demrna主要集中在寡糖结合、核苷酸结合寡聚化结构域包含一个信号通路以及肽转运ATPase活性。(4)京都基因与基因组百科全书分析显示，随着年龄的增长，demrna主要富集于类固醇生物合成、金黄色葡萄球菌感染和移植物抗宿主病中。(5) Spearman相关系数法评价NGS准确性，NGS结果与定量逆转录聚合酶链反应结果呈正相关（rs = 0.74, P < 0.05）。这些发现证实了成年大鼠和幼鼠坐骨神经基因表达的差异，提示周围神经的细胞和微环境随着年龄的增长而改变，从而影响周围神经的功能和修复。
An aging-induced decrease in Schwann cell viability can affect regeneration following peripheral nerve injury in mammals. It is therefore necessary to investigate possible age-related changes in gene expression that may affect the biological function of peripheral nerves. Ten 1-week-old and ten 12-month-old healthy male Sprague-Dawley rats were divided into young (1 week old) and adult (12 months old) groups according to their ages. mRNA expression in the sciatic nerve was compared between young and adult rats using next-generation sequencing (NGS) and bioinformatics (n = 4/group). The 18 groups of differentially expressed mRNA (DEmRNAs) were also tested by quantitative reverse transcription polymerase chain reaction (n = 6/group). Results revealed that (1) compared with young rats, adult rats had 3608 groups of DEmRNAs. Of these, 2684 were groups of upregulated genes, and 924 were groups of downregulated genes. Their functions mainly involved cell viability, proliferation, differentiation, regeneration, and myelination. (2) The gene with the most obvious increase of all DEmRNAs in adult rats was Thrsp (log2 FC = 9.01, P < 0.05), and the gene with the most obvious reduction was Col2a1 (log2FC = –8.89, P < 0.05). (3) Gene Ontology analysis showed that DEmRNAs were mainly concentrated in oligosaccharide binding, nucleotide-binding oligomerization domain containing one signaling pathway, and peptide-transporting ATPase activity. (4) Analysis using the Kyoto Encyclopedia of Genes and Genomes showed that, with increased age, DEmRNAs were mainly enriched in steroid biosynthesis, Staphylococcus aureus infection, and graft-versus-host disease. (5) Spearman's correlation coefficient method for evaluating NGS accuracy showed that the NGS results and quantitative reverse transcription polymerase chain reaction results were positively correlated (rs = 0.74, P < 0.05). These findings confirm a difference in sciatic nerve gene expression between adult and young rats, suggesting that, in peripheral nerves, cells and the microenvironment change with age, thus influencing the function and repair of peripheral nerves.