Experimental hut evaluation of bednets treated with an organophosphate (chlorpyrifos-methyl) or a pyrethroid (lambdacyhalothrin) alone and in combination against insecticide-resistant Anopheles gambiae and Culex quinquefasciatus mosquitoes -: art. no. 25
Experimental hut evaluation of bednets treated with an organophosphate (chlorpyrifos-methyl) or a pyrethroid (lambdacyhalothrin) alone and in combination against insecticide-resistant Anopheles gambiae and Culex quinquefasciatus mosquitoes -: art. no. 25
复制标题
DOI：
10.1186/1475-2875-4-25
复制
发表时间：
2005-05-26
期刊：
影响因子：
3
通讯作者：
Rowland, MW
中科院分区：
文献类型：
--
作者：
Asidi, AN;Guessan, RN;Rowland, MW
背景：对拟除虫菊酯具有抗药性的蚊子在非洲部分地区越来越普遍。必须确定替代杀虫剂，必要时可用于替代或补充驱虫蚊帐上使用的拟除虫菊酯。某些化合物的早期一代杀虫剂，有机磷可能有潜力作为净treatment.Methods：毒死蜱-甲基（CM），有机磷低哺乳动物毒性，和三氯氟氰菊酯（L），拟除虫菊酯，进行了比较研究，在实验小屋在科特迪瓦，西非。该地区的冈比亚按蚊和致倦库蚊对拟除虫菊酯和有机磷（kdr和不敏感的乙酰胆碱酯酶Ace）具有抗性。1（R））。几种治疗和应用率的完整或有孔的蚊帐进行了评估，包括单一治疗，混合物，和差异墙/天花板treatments.Results和结论：所有的治疗是有效的，减少吸血的睡眠者在蚊帐下，并杀死两种蚊子，尽管存在的kdr和ACE。1（R）基因频率较高。在大多数情况下，各种治疗的效果没有显着差异。用肥皂溶液清洗蚊帐5次，并不能减少杀虫剂对A.冈比亚的死亡率，但确实导致吸血增加。这三种组合的表现与单一杀虫剂处理没有不同，但低剂量混合物的表现令人满意，表明这种组合可用于控制杀虫剂抗性蚊子。携带Ace的蚊子的死亡率。1（R）和Ace。1（S）基因与仅携带Ace的蚊子没有显著差异。1（S）基因的任何处理网，表明Ace。1（R）等位基因在实验小屋的现实条件下不赋予对甲基毒死蜱的有效抗性。
Background: Pyrethroid resistant mosquitoes are becoming increasingly common in parts of Africa. It is important to identify alternative insecticides which, if necessary, could be used to replace or supplement the pyrethroids for use on treated nets. Certain compounds of an earlier generation of insecticides, the organophosphates may have potential as net treatments.Methods: Comparative studies of chlorpyrifos-methyl ( CM), an organophosphate with low mammalian toxicity, and lambdacyhalothrin ( L), a pyrethroid, were conducted in experimental huts in Cote d'Ivoire, West Africa. Anopheles gambiae and Culex quinquefasciatus mosquitoes from the area are resistant to pyrethroids and organophosphates ( kdr and insensitive acetylcholinesterase Ace. 1(R)). Several treatments and application rates on intact or holed nets were evaluated, including single treatments, mixtures, and differential wall/ceiling treatments.Results and Conclusion: All of the treatments were effective in reducing blood feeding from sleepers under the nets and in killing both species of mosquito, despite the presence of the kdr and Ace. 1(R) genes at high frequency. In most cases, the effects of the various treatments did not differ significantly. Five washes of the nets in soap solution did not reduce the impact of the insecticides on A. gambiae mortality, but did lead to an increase in blood feeding. The three combinations performed no differently from the single insecticide treatments, but the low dose mixture performed encouragingly well indicating that such combinations might be used for controlling insecticide resistant mosquitoes. Mortality of mosquitoes that carried both Ace. 1(R) and Ace. 1(S) genes did not differ significantly from mosquitoes that carried only Ace. 1(S) genes on any of the treated nets, indicating that the Ace. 1(R) allele does not confer effective resistance to chlorpyrifos-methyl under the realistic conditions of an experimental hut.