Noise suppression by active optics in low-dose electron microscopy
Noise suppression by active optics in low-dose electron microscopy
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DOI：
10.1063/1.2870097
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发表时间：
2008-02
影响因子：
4
通讯作者：
H. Okamoto
中科院分区：
文献类型：
--
作者：
H. Okamoto
由于噪声中固有的微弱信号，对辐射敏感的生物样品的低剂量电子显微镜只能获得有限的分辨率。在这里，我们建议通过使用类似于Castaing-Henry装置的可变形镜来控制电子波的相位来降低噪声。该方法利用了测量的量子性质和任何感兴趣的样本结构的非随机性。模拟结果表明，对于核糖体的典型案例，噪声降低了9.7%。随着对标本认识的提高，预计会有越来越好的数据。
Low-dose electron microscopy of radiation sensitive biological specimens achieves only limited resolution because of the inherently weak signal buried in noise. Here, we propose to reduce the noise by using a deformable mirror similar to the Castaing–Henry device to manipulate phase of the electron waves. The method takes advantage of quantum nature of the measurement and nonrandomness of any specimen structure of interest. The simulation revealed 9.7% reduction of noise for the representative case of the ribosome. Increasingly better figures are expected as knowledge about a specimen improves.