Characterization of a novel salt-tolerant strain Sphingopyxis sp. CY-10 capable of heterotrophic nitrification and aerobic denitrification
Characterization of a novel salt-tolerant strain Sphingopyxis sp. CY-10 capable of heterotrophic nitrification and aerobic denitrification
复制标题
新型耐盐菌株Sphingopyxis sp.的表征。
DOI：
10.1016/j.biortech.2022.127353
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发表时间：
2022-06-01
影响因子：
11.4
通讯作者：
Zhao, Tiantao
中科院分区：
文献类型：
--
作者：
Chen, Peipei;Zhang, Fupan;Zhao, Tiantao
分离到一株异养硝化好氧反硝化(HN-AD)新菌株CY-10，经鉴定为鞘氨醇属(Sphingopyxis)。以氨氮、硝酸盐和亚硝酸盐为唯一氮源(300 mg/L)时，菌株CY-10的最大脱氮率分别为100%、91.1%和68.5%。菌株CY-10去除氨氮的最佳盐度范围为0~5%。在盐度为5%时，最大脱氮率为6.25 mg/(L Middoth)。代谢组学数据表明，在5%的盐度条件下，蔗糖和D-塔格糖的代谢水平显著增加，使该菌株能够调节渗透压并在高盐环境中生存。通过定量聚合酶链式反应成功地扩增出CY-10菌株的功能基因，其HN-AD途径依次为NH4+-N-&Gt；NH2OH-&Gt；NO2--N-&Gt；NO-&Gt；N2O-&Gt；N-2。这些结果表明，菌株CY-10在含盐废水的脱氮处理中具有很大的潜力。
A novel heterotrophic nitrification and aerobic denitrification (HN-AD) strain CY-10 was isolated and identified as Sphingopyxis sp. When ammonium, nitrate or nitrite was used as the sole nitrogen source (300 mg/L), the maximum nitrogen removal efficiency of strain CY-10 were 100%, 91.1% and 68.5%, respectively. The optimal salinity for ammonia nitrogen removal by strain CY-10 was in the range of 0-5%. At the salinity of 5%, a maximum nitrogen removal rate of 6.25 mg/(Lmiddoth) was realized. Metabonomics data showed that the metabolic levels of sucrose and D-tagatose increased significantly at 5% salinity condition, enabling the strain to regulate osmotic pressure and survive in high-salt environments. Functional genes were successfully amplified by quantitative PCR, and HN-AD pathway of strain CY-10 followed NH4+-N -> NH2OH -> NO2--N -> NO -> N2O -> N-2. These findings show that strain CY-10 has great potential in nitrogen removal treatment of saline wastewater.