ION MOVEMENTS STUDIED WITH SINGLE ISOLATED ELECTROPLAX
ION MOVEMENTS STUDIED WITH SINGLE ISOLATED ELECTROPLAX
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DOI：
10.1111/j.1749-6632.1959.tb49314.x
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发表时间：
1959-01-01
期刊：
影响因子：
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通讯作者：
SCHOFFENIELS, E
中科院分区：
文献类型：
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作者：
SCHOFFENIELS, E
在过去的3年中，从电子电气器官中开发的单细胞制剂足以测量通过导电和非导电薄膜的离子通量。与1955年前使用的复杂制剂不同，新制剂只包含一个孤立的电极板。这个细胞单元分离了两个液体池，一个专门沐浴细胞的传导膜，另一个则是细胞的非导电膜。所有从一个池移动到另一个池的离子都必须通过细胞膜。新的制剂消除了以前遇到的许多限制和困难，并提供了一种有趣的、在许多方面独特的工具，用于借助高度专门用于这一功能的细胞来研究生物电流产生的物理和化学因素。给出了到目前为止观察到的一些结果。
A monocellular preparation developed during the last 3 years from the electric organ of Electrophorus electricus is adequate for measuring ion fluxes across a conducting and a nonconducting membrane. In contrast to the complex preparations used until 1955, in which pieces of electroplax were studied, the new preparation contains only a single isolated electroplax. This cellular unit separates 2 pools of fluid, the one bathing exclusively the conducting, the other the nonconducting membrane of the cell. All ions moving from one pool to the other must pass through the cellular membranes. The new preparation has eliminated many limitations and difficulties encountered previously and offers an interesting and in many respects unique tool for the study of physical and chemical factors underlying the generation of bioelectric currents with the aid of a cell highly specialized for this function. Some results thus far observed are presented.