Antigenic determinants of the membrane-bound hydrogenase in Alcaligenes eutrophus are exposed toward the periplasm

Antigenic determinants of the membrane-bound hydrogenase in Alcaligenes eutrophus are exposed toward the periplasm
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真养产碱菌膜结合氢化酶的抗原决定簇暴露于周质

DOI:
10.1128/jb.177.21.6309-6312.1995
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发表时间:
1995
影响因子:
3.2
通讯作者:
F. Mayer
F. Mayer
中科院分区:
生物学3区
文献类型:
--
作者:
K. Eismann;K. Mlejnek;D. Zipprich;M. Hoppert;H. Gerberding;F. Mayer

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用真养产碱杆菌的原生质体细胞切片和原生质体和原生质球的“整体”样品进行了电镜免疫金标记实验。他们证明了膜结合氢化酶的抗原决定簇暴露在细胞质膜外的周质中。
Electron microscopic immunogold labeling experiments were performed with ultrathin sections of plasmolyzed cells of Alcaligenes eutrophus and "whole-mount" samples of spheroplasts and protoplasts. They demonstrated that antigenic determinants of the membrane-bound hydrogenase are exposed, at the outside of the cytoplasmic membrane, to the periplasm.
使用免疫电子显微镜程序对普通脱硫弧菌的膜相关 (NiFe) 和 (NiFeSe) 氢化酶进行定位。
DOI: 10.1111/j.1432-1033.1990.tb19134.x
发表时间: 1990
期刊: European journal of biochemistry
影响因子: --
作者:
Rohde,M;Fürstenau,U;Mayer,F;Przybyla,AE;PeckJr,HD;LeGall,J;Choi,ES;Menon,NK
通讯作者: Menon,NK