Multiplexed molecular descriptors of pressure ulcers defined by imaging mass spectrometry.
Multiplexed molecular descriptors of pressure ulcers defined by imaging mass spectrometry.
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通过成像质谱定义的压疮的多重分子描述符。
DOI：
10.1111/j.1524-475x.2011.00738.x
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Caprioli,Richard
中科院分区：
文献类型：
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作者：
Taverna,Domenico;Nanney,LillianB;Pollins,AlondaC;Sindona,Giovanni;Caprioli,Richard
压疮愈合受损的发病机制仍然不清楚，很少检查。我们描述了一项试点研究的结果，该研究应用基质辅助激光解吸/电离成像质谱技术进行直接组织分析，以评估伤口微环境中焦点区域2至20 kDa的蛋白质组学特征和300-1,200 Da的磷脂。区分溃疡上、下区域的分子差异是明显的，并进一步通过蛋白质谱、离子密度图、主成分分析和微阵列显著性分析与邻近真皮和表皮边缘进行对比。一些以前在压疮中未被发现的蛋白质，如α -防御素（人中性粒细胞肽[HNP]‐1，‐2，‐3），是指示伤口状态是改善还是在有害的慢性状态下延长的潜在标志物。胸腺蛋白酶β4似乎是一个有利的蛋白质标记物，在邻近的真皮和伤口床的成熟区域显示出较高的相对水平。脂质组学检查显示存在主要的脂类：甘油酰胆碱、甘油酰甘油、甘油酰肌醇和三酰基甘油。我们从蛋白质组学或脂质组学特征或个体分布的全局角度检查了试点数据，结果表明成像质谱技术可以有效地用于慢性伤口微环境中分子干扰的发现和空间定位。
The pathogenesis of impaired healing within pressure ulcers remains poorly characterized and rarely examined. We describe the results of a pilot study that applies matrix‐assisted laser desorption/ionization imaging mass spectrometry technology for direct tissue analysis to evaluate proteomic signatures ranging from 2 to 20 kDa and phospholipids from 300–1,200 Da in focal regions within the wound microenvironment. Distinguishing molecular differences were apparent between upper vs. lower regions of ulcers and further contrasted against adjacent dermis and epidermal margins using protein profiles, ion density maps, principal component analysis and significant analysis of microarrays. Several proteins previously uncharacterized in pressure ulcers, the α‐defensins (human neutrophil peptide [HNP]‐1, ‐2, ‐3), are potential markers indicating whether the wound status is improving or being prolonged in a deleterious, chronic state. Thymosin β4 appears to be a favorable protein marker showing higher relative levels in adjacent dermis and maturing areas of the wound bed. Lipidomic examination revealed the presence of major lipid classes: glycerophosphocholines, glycerophosphoglycerols, glycerophosphoinositols, and triacylglycerols. Our pilot data examined from either a global perspective using proteomic or lipidomic signatures or as individual distributions reveal that imaging mass spectrometry technology can be effectively used for discovery and spatial mapping of molecular disturbances within the microenvironment of chronic wounds.