RAT INSULIN GENES - CONSTRUCTION OF PLASMIDS CONTAINING CODING SEQUENCES
RAT INSULIN GENES - CONSTRUCTION OF PLASMIDS CONTAINING CODING SEQUENCES
复制标题
DOI：
10.1126/science.325648
复制
发表时间：
1977-01-01
期刊：
影响因子：
56.9
通讯作者：
GOODMAN, HM
中科院分区：
文献类型：
--
作者：
ULLRICH, A;SHINE, J;GOODMAN, HM
构建含有从大鼠胰岛信使RNA制备的互补DNA的重组细菌质粒。三种质粒含有代表大鼠胰岛素原I的完整编码区、前胰岛素原I前肽的一部分和mRNA的非翻译3“末端区的克隆序列。第4个质粒含有衍生自大鼠前胰岛素原II的A链区的序列。
Recombinant bacterial plasmids were constructed that contain complementary DNA prepared from rat islets of Langerhans messenger RNA. Three plasmids contain cloned sequences representing the complete coding region of rat proinsulin I, part of the preproinsulin I prepeptide, and the untranslated 3'' terminal region of the mRNA. A 4th plasmid contains sequences derived from the A chain region of rat preproinsulin II.