Carbon-13 and phosphorus-31 nuclear magnetic resonance studies on interaction of calcium with phosphatidylserine.
Carbon-13 and phosphorus-31 nuclear magnetic resonance studies on interaction of calcium with phosphatidylserine.
复制标题
钙与磷脂酰丝氨酸相互作用的碳 13 和磷 31 核磁共振研究。
DOI：
10.1021/bi00505a030
复制
发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Wuthier,RE
中科院分区：
文献类型：
--
作者：
Holwerda,DL;Ellis,PD;Wuthier,RE
丹尼斯湖保罗·霍尔韦达Ellis和Roy E. Wuthier* 摘要：钙离子和磷脂酰丝氨酸之间的相互作用进行了研究，通过13 C和31 P NMR光谱，IR分析，结合常数的测量，并通过使用空间填充分子模型。在两种类型的有机溶剂中对脂质的各种盐形式进行NMR测量，这两种溶剂允许充分平均化学位移各向异性和偶极耦合以产生高分辨率光谱。极性头基碳的13 C共振相对于酰基链的那些被加宽。在中性pH下制备的样品中尤其如此，其中离子相互作用似乎限制了分子运动。在CDC 13中，以Ca 2+形式存在的极性头基原子的共振谱线明显变宽，表明形成了大的、缓慢翻滚的胶束。在两亲性溶剂中，谱线展宽的大幅减少表明存在自由翻滚的Ca-（磷脂酰丝氨酸）2二聚复合物。第二：1结合化学计量和Ca-磷脂酰丝氨酸复合物的低化学活性支持了这一点（长期以来已知钙与磷脂酰丝氨酸结合（Nash & Tobias，1964; Abramson等人，1964; Hendrickson & Fullington，1965），并且这种相互作用被认为在多种生物过程中具有生理学意义。除了众所周知的Ca 2+在凝血（Davie & Fujikawa，1975）和各种软组织膜相关过程中与磷脂结合的功能之外，已显示Ca 2+在矿化组织中与磷脂酰丝氨酸和其他酸性磷脂复合[例如，Wuthier（1968，1971），Eisenberget al.（1970）和Boskey & Posner（1976）]。无机磷酸盐已经显示出定量地增强Ca 2+与磷脂酰丝氨酸的结合（Cotmore等人，（1971年）导致
Dennis L. Holwerda, Paul D. Ellis, and Roy E. Wuthier* abstract: The interaction between Ca2+ and phosphatidylserine was studied by 13C and 31P NMR spectroscopy, by IR analysis, by binding constant measurements, and through use of space-filling molecular models. NMR measurements of various salt forms of the lipid were made in two types of organic solvents that allowed sufficient averaging of chemical shift anisotropy and dipolar couplingsto yield high resolution spectra. 13C resonances of the polar head-group carbons were broadened relative to those of the acyl chains. This was es-pecially true in samples prepared at neutral pH where ionic interactions appeared to restrict molecular motion. In CDC13 the marked line broadening of the resonances of the polar head-group atoms in the Ca2+ form indicated the formation of large, slow tumbling micelles. In the amphipathic solvent the large reduction in line broadening indicated the presence of freely tumbling Ca-(phosphatidylserine) 2 dimeric com-plexes. The 2: 1 binding stoichiometry and the low chemical activity of the Ca-phosphatidylserine complex support this (Calcium has long been known to bind with phosphatidyl-serine (Nash & Tobias, 1964; Abramson et al., 1964; Hendrickson & Fullington, 1965), and such interaction is con-sidered to be of physiological significance in a variety of biological processes. In addition to the well-knownfunction of Ca2+ binding to phospholipids in blood clotting (Davie & Fujikawa, 1975) and in various soft-tissue membrane-asso-ciated processes, Ca2+ has been shown to be complexed with phosphatidylserine and other acidic phospholipids in mineralizing tissues [eg, Wuthier (1968, 1971), Eisenberget al.(1970), and Boskey & Posner (1976)]. Inorganic phosphate has been shown to enhance quantitatively the binding of Ca2+ to phosphatidylserine (Cotmore et al., 1971) leading to the