WAVELENGTHS EFFECTIVE IN INDUCTION OF MALIGNANT-MELANOMA
WAVELENGTHS EFFECTIVE IN INDUCTION OF MALIGNANT-MELANOMA
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DOI：
10.1073/pnas.90.14.6666
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发表时间：
1993-07-15
影响因子：
11.1
通讯作者：
WOODHEAD, AD
中科院分区：
文献类型：
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作者：
SETLOW, RB;GRIST, E;WOODHEAD, AD
人们普遍认为，阳光照射是白皮肤个体恶性黑色素瘤的病因之一。然而，导致肿瘤发生的波长尚不清楚，尽管 DNA 被认为是目标，因为 DNA 紫外线损伤修复缺陷的个体比普通人群更容易患上这种疾病数千倍。剑尾鱼属（鸭嘴鱼和剑尾鱼）的色素重的回交杂交种对单次暴露于紫外线下诱导黑色素瘤非常敏感。我们用 302、313、365、405 和 436 nm 的窄波长带照射了五组 6 日龄的鱼，并在 4 个月后对受照射动物的黑色素瘤进行评分。我们在每个波长下进行多次曝光，以获得作为曝光和波长函数的黑色素瘤诱导敏感性的估计值。黑色素瘤诱导的作用光谱（每个入射光子的灵敏度作为波长的函数）在 365、405 和可能 436 nm 处显示出明显的灵敏度，这表明未直接被 DNA 吸收的波长在诱导中是有效的。我们将结果解释为表明黑色素吸收的光能可有效诱导该动物模型中的黑色素瘤，并且在自然阳光下，90-95% 的黑色素瘤诱导可能归因于波长 > 320 nm（UV-A 和可见光谱区域）。
It is generally agreed that sunlight exposure is one of the etiologic agents in malignant melanoma of fair-skinned individuals. However, the wavelengths responsible for tumorigenesis are not known, although DNA is assumed to be the target because individuals defective in the repair of UV damage to DNA are several thousandfold more prone to the disease than the average population. Heavily pigmented backcross hybrids of the genus Xiphophorus (platyfish and swordtails) are very sensitive to melanoma induction by single exposures to UV. We irradiated groups of five 6-day-old fish with narrow wavelength bands at 302, 313, 365, 405, and 436 nm and scored the irradiated animals for melanomas 4 months later. We used several exposures at each wavelength to obtain estimates of the sensitivity for melanoma induction as a function of exposure and wavelength. The action spectrum (sensitivity per incident photon as a function of wavelength) for melanoma induction shows appreciable sensitivity at 365, 405, and probably 436 nm, suggesting that wavelengths not absorbed directly in DNA are effective in induction. We interpret the results as indicating that light energy absorbed in melanin is effective in inducing melanomas in this animal model and that, in natural sunlight, 90-95% of melanoma induction may be attributed to wavelengths > 320 nm-the UV-A and visible spectral regions.