Specificity and mechanism of antitermination by Q proteins of bacteriophages lambda and 82.
Specificity and mechanism of antitermination by Q proteins of bacteriophages lambda and 82.
复制标题
噬菌体 lambda 和 82 的 Q 蛋白抗终止的特异性和机制。
DOI：
10.1016/0022-2836(89)90122-8
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发表时间：
1989
影响因子：
5.6
通讯作者：
Roberts,JW
中科院分区：
文献类型：
--
作者：
Yang,XJ;Goliger,JA;Roberts,JW
Lambdoid 噬菌体晚期基因操纵子受到每个噬菌体 Q 基因编码的基因组特异性抗终止子蛋白的正向调节。在本文中，我们比较了噬菌体 λ 和噬菌体 82 Q 蛋白的活性。 Q82 介导的抗终止作用与 Qλ 一样，涉及转录暂停，在此期间调节因子可以修改 RNA 聚合酶。我们表明，Q82 和 Qλ 的活性在将 RNA 聚合酶从早期暂停位点中追逐出来并介导抗终止方面都是基因组特异性的。最后，我们证明暂停复合物中 RNA 的长度或暂停的确切位置会影响 Q82 将 RNA 聚合酶追出暂停的效率。
Lambdoid phage late gene operons are positively regulated by genome-specific antiterminator proteins encoded by theQgene of each phage. In this paper, we compare the activity of phage λ and phage 82 Q proteins. Q82-mediated antitermination, like that of Qλ, involves a transcription pause during which the regulator can modify RNA polymerase. We show that the activities of both Q82and Qλare genome-specific in chasing RNA polymerase out of the early pause sites and in mediating antitermination. Finally, we show that the length of the RNA in the paused complex, or the exact position of the pause, affects the efficiency with which Q82chases RNA polymerase out of the pause.