Study on performance of magnetic fluorescent nanoparticles as gene carrier and location in pig kidney cells.
Study on performance of magnetic fluorescent nanoparticles as gene carrier and location in pig kidney cells.
复制标题
DOI：
10.1186/1556-276x-8-127
复制
发表时间：
2013-03-15
影响因子：
--
通讯作者：
Zhao X
中科院分区：
文献类型：
--
作者：
Wang Y;Cui H;Sun C;Du W;Cui J;Zhao X
我们研究了绿色荧光磁性Fe3O4纳米颗粒（NPs）作为基因载体和定位在猪肾细胞中的性能。当NPs与绿色荧光蛋白质粒DNA的质量比达到1:16以上时，DNA分子可以与NPs完全结合，说明NPs具有良好的结合阴性DNA的能力。原子力显微镜（AFM）实验研究了NPs与DNA的结合机制。原子力显微镜（AFM）图像显示，单个DNA链从更大的网状团块上脱落，几个球形纳米颗粒附着在每个DNA链上，并相互作用。用膜特异性红色荧光染料1,1′-二十八烷基-3,3,3′，3′-四甲基多碳菁高氯酸盐和核特异性蓝色荧光染料4′，6-二氨基-2-苯基吲哚二盐酸盐标记猪肾细胞。我们发现绿色荧光纳米颗粒可以穿过细胞膜，在细胞内部扩散。NPs似乎更多地位于细胞质中而不是细胞核中。
We evaluated the performance of green fluorescent magnetic Fe3O4 nanoparticles (NPs) as gene carrier and location in pig kidney cells. When the mass ratio of NPs to green fluorescent protein plasmid DNA reached 1:16 or above, DNA molecules can be combined completely with NPs, which indicates that the NPs have good ability to bind negative DNA. Atomic force microscopy (AFM) experiments were carried out to investigate the binding mechanism between NPs and DNA. AFM images show that individual DNA strands come off of larger pieces of netlike agglomerations and several spherical nanoparticles are attached to each individual DNA strand and interact with each other. The pig kidney cells were labelled with membrane-specific red fluorescent dye 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate and nucleus-specific blue fluorescent dye 4′,6-diamidino-2-phenylindole dihydrochloride. We found that green fluorescent nanoparticles can past the cell membrane and spread throughout the interior of the cell. The NPs seem to locate more frequently in the cytoplasm than in the nucleus.