Red blood cell deformability in sepsis
Red blood cell deformability in sepsis
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DOI：
10.1164/ajrccm.157.2.9611103
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发表时间：
1998-02-01
影响因子：
24.7
通讯作者：
Meiselman, HJ
中科院分区：
文献类型：
--
作者：
Baskurt, OK;Gelmont, D;Meiselman, HJ
脓毒症的微循环障碍促使对红细胞(RBC)机械行为(即变形性)的微孔批量过滤研究。然而，这些先前的报告可能并不完全反映RBC的行为，因为可能是白细胞(WBC)阻塞了滤孔。本研究旨在使用不受WBC伪影影响的技术检查人类和实验性脓毒症的RBC机械改变。红细胞来自成人败血症患者和健康对照供者。采用盲肠结扎穿刺法建立实验性脓毒症模型，从瑞士大白鼠身上获取红细胞。用计算机微孔过滤系统(CTA)和激光衍射仪(LORCA)测试了红细胞的力学行为，后者提供了红细胞在不同应力下的变形程度和红细胞形状恢复的时间常数。显著的发现包括：(1)人红细胞在流体剪切力<；5pa(LOCA)下的变形性显著降低，但与CTA对照组无差异；(2)实验性脓毒症大鼠RBC的变形性和形状恢复时间常数(LOCA)显著降低，但与CTA无差异。我们得出结论，脓毒症患者的红细胞变形性降低，但微孔滤过法可能不适合检测这些变化。
The microcirculatory disturbances in sepsis have prompted micropore bulk-filtration studies of red blood cell (RBC) mechanical behavior (i.e., deformability). However, these prior reports may not solely reflect RBC behavior because of possible white blood cell (WBC) occlusion of the filter pores. The present study was designed to examine RBC mechanical alterations in human and experimental sepsis using techniques that are not affected by WBC artifacts. RBC were obtained from adult patients with sepsis and from healthy control donors. RBC were also obtained from Swiss-albino rats in which experimental sepsis was induced via cecal ligation-puncture. Red cell mechanical behavior was tested using a computerized micropore filtration system (CTA) and a laser-diffraction shearing device (LORCA); the latter provides the extent of RBC deformation at various stresses and the time constant for RBC shape recovery. Salient findings include: (1) for human RBC, significantly decreased deformability at fluid shear stresses < 5 Pa (LORCA) yet no differences from control with the CTA; (2) for rat RBC in experimental sepsis, significant decreases of deformability and shape-recovery time constant (LORCA) but no differences with the CTA. We conclude that RBC deformability is reduced in sepsis but that micropore bulk-filtration methods may not be appropriate for detecting these changes.