Generation of solution and surface gradients using microfluidic systems
Generation of solution and surface gradients using microfluidic systems
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DOI：
10.1021/la000600b
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发表时间：
2000-10-31
期刊：
影响因子：
3.9
通讯作者：
Whitesides, GM
中科院分区：
文献类型：
--
作者：
Jeon, NL;Dertinger, SKW;Whitesides, GM
本文描述了一种简单，通用的方法产生梯度的组成在溶液或表面上使用微流体系统。这种方法是基于在微通道网络内以低雷诺数层流的溶液中控制物质的扩散混合。我们演示了使用该程序来产生(1)溶液组成的梯度，直接通过比色法测定；(2)通过产生蚀刻试剂的浓度梯度产生的表面形貌梯度，然后使用这些梯度将轮廓蚀刻到基板上。这里检查的梯度的横向尺寸，从350到900毫米，是由微通道的宽度决定的。利用该方法生成了不同大小、分辨率和形状的梯度。通过改变输入流体流的相对流速，可以连续改变梯度的形状（动态梯度）。该方法在实验上简单，适应性强，不需要特殊设备，除了可以通过快速原型制作的弹性体浮雕结构。这项技术为研究依赖于浓度梯度的现象提供了一个新的平台，特别是细胞生物学（趋化性和趋合性）和表面化学（晶体成核和生长、蚀刻和马兰戈尼效应）中的动态现象。
This paper describes a simple, versatile method of generating gradients in composition in solution or on surfaces using microfluidic systems. This method is based on controlled diffusive mixing of species in solutions that are flowing laminarly, at low Reynolds number, inside a network of microchannels. We demonstrate the use of this procedure to generate (1) gradients in the compositions of solutions, measured directly by colorimetric assays and (2) gradients in topography of the surfaces produced by generating concentration gradients of etching reagents, and then using these gradients to etch profiles into the substrate. The lateral dimensions of the gradients examined here, which went from 350 to 900 mum, are determined by the width of the microchannels. Gradients of different size, resolution, arid shape have been generated using this method. The shape of the gradients can be changed continuously (dynamic gradients) by varying the relative flow velocities of the input streams of fluids. The method is experimentally simple and highly adaptable, and requires no special equipment except for an elastomeric relief structure that can be readily prepared by rapid prototyping. This technique provides a new platform with which to study phenomena that depend on gradients in concentration, especially dynamic phenomena in cell biology (chemotaxis and haptotaxis) and surface chemistry (nucleation and growth of crystals, etching, and Marangoni effects).