Sequence, Secondary Structure, and Phylogenetic Analyses of the Ribosomal Internal Transcribed Spacer 2 (ITS2) in Members of the North American Signifera Group of Orthopodomyia (Diptera: Culicidae)
Sequence, Secondary Structure, and Phylogenetic Analyses of the Ribosomal Internal Transcribed Spacer 2 (ITS2) in Members of the North American Signifera Group of Orthopodomyia (Diptera: Culicidae)
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DOI：
10.1603/me10243
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发表时间：
2012-11
期刊：
影响因子：
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通讯作者：
B. Byrd;B. A. Harrison;T. Zavortink;D. Wesson
中科院分区：
文献类型：
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作者：
B. Byrd;B. A. Harrison;T. Zavortink;D. Wesson
摘要直足蚊属（双翅目：蚊科）是一种鲜为人知的蚊类，其流行病学意义尚不明确。在美国，有三种Orthopodomyia物种（即，或. signifiera（Coquillett），Or. Alba Baker和Or.根据目前的形态学分类，它们都是Signifera组的成员。在对新采集的标本进行鉴定的过程中，发现目前用于分离四龄幼虫的关键形态特征存在问题。signifera和Or.我是阿西米获得rDNA的内转录间隔区两个序列以解析身份。Orthopodomyia internal transcribed spacer 2的大小范围为193（Or. 244 bp（Or. signifera）（平均值= 218 bp），并且稍微富含腺嘌呤/胸腺嘧啶（平均44.7%鸟嘌呤/胞嘧啶）。推定的二级结构揭示了物种之间一致的结构同源性（四个结构域），这些结构域还具有蚊子特有的保守序列（例如，最长螺旋3 ′端的保守基序：GARTACATCC）。序列分析表明，在北美西南部的某些地区，Or之间可能发生杂交。马林和或。signifera。此外，我们的分析证实，或。califomica（英语：califomica）是Or.”（《易经》）或“或”。符号。据我们所知，这是第一个基于序列的系统发育和分子分析的Orthopodomyia。
ABSTRACT Mosquitoes of the genus Orthopodomyia (Diptera: Culicidae) are little known and of uncertain epidemiological importance. In the United States, there are three Orthopodomyia species (i.e., Or. signifiera (Coquillett), Or. alba Baker, and Or. kummi Edwards); they are all members of the Signifera Group based on the current morphological taxonomy. In the course of identifying recently collected specimens, a problem was found with the current key morphological characters for separating the fourth instar larvae of Or. signifera and Or. kummi. Internal transcribed spacer two sequences of the rDNA were obtained to resolve the identities. The Orthopodomyia internal transcribed spacer two ranged in size from 193 (Or. kummi) to 244 bp (Or. signifera) (mean = 218 bp) and were slightly Adenine/Thymine enriched (44.7% Guanine/Cytosine on average). Putative secondary structures reveal structural homologies (four domains) consistent between species that also feature conserved sequences specific to mosquitoes (e.g., a conserved motif on the 3′ aspect of the longest helix: GARTACATCC). Sequence analyses suggest that in certain areas of southwestern North America, hybridization may occur between Or. kummi and Or. signifera. Furthermore, our analyses confirm that Or. califomica (a junior synonym of Or. signifera) is indeed Or. signifera. To our knowledge, this is the first sequence-based phylogenetic and molecular analysis of the Orthopodomyia.