Constriction of the mitochondrial inner compartment is a priming event for mitochondrial division.
Constriction of the mitochondrial inner compartment is a priming event for mitochondrial division.
复制标题
DOI：
10.1038/ncomms15754
复制
发表时间：
2017-06-09
影响因子：
16.6
通讯作者：
Sun W
中科院分区：
文献类型：
--
作者：
Cho B;Cho HM;Jo Y;Kim HD;Song M;Moon C;Kim H;Kim K;Sesaki H;Rhyu IJ;Kim H;Sun W
线粒体分裂对于维持和调节线粒体的功能、质量和分布至关重要。这一过程由线粒体外膜（OMM）上的基于细胞质肌动蛋白的收缩机制和动力蛋白相关蛋白1（Drp 1）控制。虽然线粒体生理学，包括氧化磷酸化，也是重要的有效的线粒体分裂，线粒体内膜（IMM）的形态学改变尚未明确阐明。在这里，我们报告自发和重复收缩线粒体内室（CoMIC）与随后的分裂神经元。虽然CoMIC是通过抑制Drp 1而增强的，并且发生在与内质网接触的潜在分裂点，但它独立于OMM出现在IMM上。线粒体内Ca 2+内流诱导并增强CoMIC，并导致K+介导的线粒体膨胀和去极化。协同地，视神经萎缩1（Opa 1）也通过控制Mic 60介导的OMM-IMM系留来调节CoMIC。因此，我们提出，CoMIC是一个启动事件，有效的线粒体分裂。线粒体内膜的形态学改变在调节线粒体分裂中的作用尚不清楚。在这里，作者描述了线粒体内室的自发和重复收缩，并认为这是有效线粒体分裂的启动事件。
Mitochondrial division is critical for the maintenance and regulation of mitochondrial function, quality and distribution. This process is controlled by cytosolic actin-based constriction machinery and dynamin-related protein 1 (Drp1) on mitochondrial outer membrane (OMM). Although mitochondrial physiology, including oxidative phosphorylation, is also important for efficient mitochondrial division, morphological alterations of the mitochondrial inner-membrane (IMM) have not been clearly elucidated. Here we report spontaneous and repetitive constriction of mitochondrial inner compartment (CoMIC) associated with subsequent division in neurons. Although CoMIC is potentiated by inhibition of Drp1 and occurs at the potential division spots contacting the endoplasmic reticulum, it appears on IMM independently of OMM. Intra-mitochondrial influx of Ca2+ induces and potentiates CoMIC, and leads to K+-mediated mitochondrial bulging and depolarization. Synergistically, optic atrophy 1 (Opa1) also regulates CoMIC via controlling Mic60-mediated OMM–IMM tethering. Therefore, we propose that CoMIC is a priming event for efficient mitochondrial division. The role of morphological alterations in the mitochondrial inner-membrane in regulating mitochondrial division are unknown. Here, the authors describe spontaneous and repetitive constriction of the mitochondrial inner compartment, and suggest this acts as a priming event for efficient mitochondrial division.