Glucose-sensitivity of glucose oxidase-containing cationic copolymer hydrogels having poly(ethylene glycol) grafts
Glucose-sensitivity of glucose oxidase-containing cationic copolymer hydrogels having poly(ethylene glycol) grafts
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DOI：
10.1016/s0168-3659(00)00195-4
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发表时间：
2000-06-15
影响因子：
10.8
通讯作者：
Peppas, NA
中科院分区：
文献类型：
--
作者：
Podual, K;Doyle, FJ;Peppas, NA
通过组成单体和功能化酶溶液的共聚，将葡萄糖氧化酶和过氧化氢酶固定在聚（甲基丙烯酸二乙氨基乙酯-g-乙二醇）凝胶上。水凝胶被制备成圆盘和微粒的形式。测定固定在基质中的酶的量和活性。通过将微粒暴露于不同浓度的葡萄糖来测试水凝胶对葡萄糖的响应。研究了葡萄糖与氧反应产生的葡萄糖酸与聚合物参数（例如交联比和酶负载量）的函数关系。使用葡萄糖浓度的脉动变化来确认这些水凝胶的葡萄糖依赖性溶胀特性。 (C) 2000 Elsevier Science B.V. 保留所有权利。
Glucose oxidase and catalase were immobilized on poly(diethylaminoethyl methacrylate-g-ethylene glycol) gels by copolymerization of the constituent monomers and the functionalized enzyme solutions. The hydrogels were prepared in the form of discs and microparticles. The amount and the activity of enzymes immobilized in the matrix were determined. The hydrogels were tested for their response to glucose by exposing microparticles to varying concentrations of glucose. The generation of gluconic acid as a result of the reaction of glucose with oxygen was investigated as a function of polymer parameters, such as crosslinking ratio and enzyme loading. Pulsatile variation of the glucose concentration was used to confirm the glucose-dependent swelling properties of these hydrogels. (C) 2000 Elsevier Science B.V. All rights reserved.