First-generation monoclonal antibodies identifying the human leukotriene B(4) receptor-1.
First-generation monoclonal antibodies identifying the human leukotriene B(4) receptor-1.
复制标题
识别人类白三烯 B(4) 受体 1 的第一代单克隆抗体。
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
C. Owman
中科院分区：
文献类型：
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作者：
A. Pettersson;Å. Boketoft;A. Sabirsh;N. Nilsson;K. Kotarsky;B. Olde;C. Owman
The leukotriene B(4) receptor (BLTR) is a seven-transmembrane chemoattractant receptor that is important in pro-inflammatory responses. We have produced the first widely applicable monoclonal antibodies against the human BLTR and confirmed the antibody specificity using flow cytometric analysis of three different cell lines stably expressing the recombinant receptor. The antibodies did not cross-react with the recently cloned second LTB(4) receptor, BLTR2, or the Cys LT1 and Cys LT2 receptors. Functional analysis in combination with two-color flow cytometry showed that the BLTR antibodies bind to cells that are activated by LTB(4). The antibodies were shown to recognize BLTR in cell ELISA and immunocytochemistry. Endogenous expression of BLTR in CD15-positive blood leukocytes and in differentiated HL-60 cells was also demonstrated with the antibodies.
DOI：
10.1016/s0162-3109(00)00201-0
发表时间：
2000-07
期刊：
Immunopharmacology
影响因子：
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作者：
V. Dasari;Jianguo Jin;S. Kunapuli
通讯作者：
V. Dasari;Jianguo Jin;S. Kunapuli