Neoadjuvant interferon alfa-2b treatment in a murine model for metastatic ocular melanoma - A preliminary study
Neoadjuvant interferon alfa-2b treatment in a murine model for metastatic ocular melanoma - A preliminary study
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DOI：
10.1001/archopht.118.8.1085
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发表时间：
2000-08-01
影响因子：
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通讯作者：
Grossniklaus, HE
中科院分区：
文献类型：
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作者：
Dithmar, S;Rusciano, D;Grossniklaus, HE
目的：为探讨葡萄膜黑色素瘤转移的治疗方法及干扰素α-2b（IFN α-2b）对转移瘤的治疗作用，将B16-LS9黑色素瘤细胞接种于3组C57 BL/6小鼠眼内后室。在接种后9天摘除接种的眼睛，并在接种后26天对小鼠实施安乐死;使用标准组织学技术确定转移的部位和数量。第1组为对照组，第2组于去核前12 h肌注IFN α-2b 20 000 IU，第3组于去核前4 d开始肌注IFN α-2b 20 000 IU，每日1次。第1、2和3组中分别有57%、33%和0%检测到肺转移;仅在第1组中检测到肝微转移。这些结果表明，接受IFN α-2b的小鼠肝转移与对照组相比显著减少（P = 0.005）。结论：在小鼠模型中，用IFN α-2b治疗导致眼内黑色素瘤肝转移减少。
Objectives: To investigate the treatment of metastasis from uveal melanoma and to test the effect of interferon (IFN) alfa-2b in a murine model.Methods: The B16-LS9 tissue culture melanoma cells were inoculated into the posterior intraocular compartment of 3 groups of C57BL/6 mice. The inoculated eyes were enucleated at 9 days and the mice were euthanized at 26 days after inoculation; the site and number of metastases were determined using standard histologic techniques. Group 1 was the control group; group 2 was given 20 000 international units (IU) of IFN alfa-2b intramuscularly 12 hours before enucleation, and group 3 received daily injections of 20 000 IU of IFN alfa-2b intramuscularly starting 4 days before enucleation.Results: Pulmonary metastases were detected in 57%, 33%, and 0% of groups 1, 2, and 3, respectively; hepatic micrometastases were detected only in group 1. These results showed a significant decrease in hepatic metastases in mice receiving IFN alfa-2b vs controls (P=.005).Conclusion: Treatment with IFN alfa-2b results in decreased hepatic metastases from intraocular melanoma in a murine model.