Recognition and sensing of low-epitope targets via ternary complexes with oligonucleotides and synthetic receptors.
Recognition and sensing of low-epitope targets via ternary complexes with oligonucleotides and synthetic receptors.
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作者：
基于寡核苷酸的受体或适体可以与小分子相互作用，但是实现这些相互作用的高亲和力和选择性的能力强烈地依赖于结合靶标所展示的官能团或表位。某些类别的目标是特别具有挑战性的：例如，单糖具有稀缺的功能，并且没有适体被报道识别，更不用说彼此区分葡萄糖和其他己糖。在这里，我们报告的适体，区分低表位目标，如葡萄糖，果糖，或半乳糖形成三元复合物与高表位有机受体的单糖。在后续的例子中，我们扩展了这种方法，以分离高亲和力的寡核苷酸对芳香族氨基酸与非特异性有机金属受体在原位复合。该方法是通用的，并且能够在混合和测量测定中广泛地临床使用适体来检测小分子，如通过监测人类受试者中苯丙氨酸的餐后波所证明的。
Oligonucleotide-based receptors or aptamers can interact with small molecules, but the ability to achieve high-affinity and selectivity of these interactions depends strongly on functional groups or epitopes displayed by the binding targets. Some classes of targets are particularly challenging: for example, monosaccharides have scarce functionalities and no aptamers have been reported to recognize, let alone distinguish from each other, glucose and other hexoses. Here we report aptamers that differentiate low-epitope targets such as glucose, fructose, or galactose by forming ternary complexes with high-epitope organic receptors for monosaccharides. In a follow-up example, we expand this method to isolate high-affinity oligonucleotides against aromatic amino acids complexed in situ with a non-specific organometallic receptor. The method is general and enables broad clinical use of aptamers for detection of small molecules in mix-and-measure assays, as demonstrated by monitoring postprandial waves of phenylalanine in human subjects.
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影响因子：
3.7
作者：
Gold L;Ayers D;Bertino J;Bock C;Bock A;Brody EN;Carter J;Dalby AB;Eaton BE;Fitzwater T;Flather D;Forbes A;Foreman T;Fowler C;Gawande B;Goss M;Gunn M;Gupta S;Halladay D;Heil J;Heilig J;Hicke B;Husar G;Janjic N;Jarvis T;Jennings S;Katilius E;Keeney TR;Kim N;Koch TH;Kraemer S;Kroiss L;Le N;Levine D;Lindsey W;Lollo B;Mayfield W;Mehan M;Mehler R;Nelson SK;Nelson M;Nieuwlandt D;Nikrad M;Ochsner U;Ostroff RM;Otis M;Parker T;Pietrasiewicz S;Resnicow DI;Rohloff J;Sanders G;Sattin S;Schneider D;Singer B;Stanton M;Sterkel A;Stewart A;Stratford S;Vaught JD;Vrkljan M;Walker JJ;Watrobka M;Waugh S;Weiss A;Wilcox SK;Wolfson A;Wolk SK;Zhang C;Zichi D
通讯作者：
Zichi D
影响因子：
3.6
作者：
RAMPINI, S;ANDERS, PW;MARTHALER, T
通讯作者：
MARTHALER, T
与 L-色氨酸结合的 DNA 适体的表征和应用
影响因子：
4.2
作者：
Yang, Xiaojuan;Bing, Tao;Shangguan, Dihua
通讯作者：
Shangguan, Dihua
影响因子：
3.3
作者：
Larkin, Joseph D.;Frimat, Karine A.;James, Tony D.
通讯作者：
James, Tony D.
DOI：
10.1002/anie.199422071
发表时间：
1994-11-17
期刊：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION IN ENGLISH
影响因子：
--
作者：
JAMES, TD;SANDANAYAKE, KRAS;SHINKAI, S
通讯作者：
SHINKAI, S