The monolayer formation of Bergmann glial cells is regulated by Notch/RBP-J signaling
The monolayer formation of Bergmann glial cells is regulated by Notch/RBP-J signaling
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DOI：
10.1016/j.ydbio.2007.08.042
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发表时间：
2007-11-01
影响因子：
2.7
通讯作者：
Tanaka, Kohichi
中科院分区：
文献类型：
--
作者：
Komine, Okiru;Nagaoka, Mai;Tanaka, Kohichi
Bergmann胶质细胞是小脑皮质中的单极星形胶质细胞，与浦肯野细胞紧密相关。Bergmann神经胶质细胞的胞体位于浦肯野细胞胞体周围的一排;它们延伸出放射状排列的Bergmann纤维，这些纤维包裹着浦肯野细胞树突上的突触。众所周知，Bergmann胶质细胞体从脑室区迁移通过外套带，在发育过程中在浦肯野细胞层中形成上皮样衬里。然而，Bergmann胶质细胞单层形成的机制知之甚少。一些报告表明，Notch信号在促进几种类型的神经胶质细胞，包括Bergmann神经胶质细胞的身份中起着指导性作用。而且。Notch受体在发育过程中在Bergmann神经胶质中表达。在此，我们通过GFAP驱动的Cre介导的重组删除Bergmann胶质细胞中的Notch 1、Notch 2和RBP-J基因，以研究Notch-RBP-J信号在Bergmann胶质细胞单层形成中的作用。Notch 1/2-和RBP-J-条件突变小鼠表现出伯格曼纤维的紊乱，伯格曼神经胶质衬里的不规则性和伯格曼神经胶质在分子层中的异常定位。因此，Notch-RBP-J信号在Bergmann胶质细胞单层形成和形态发生中起着至关重要的作用。(C)2007年爱思唯尔公司All rights reserved.
The Bergmann glia is a unipolar astrocyte in the cerebellar cortex, displaying a tight association with Purkinje cells. The cell bodies of Bergmann glia are located in a row around Purkinje cell somata; they extend radially arranged Bergmann fibers which enwrap the synapses on the Purkinje cell dendrites. It is well known that Bergmann glial somata migrate from the ventricular zone through the mantle zone, forming an epithelium-like lining in the Purkinje cell layer during development. However, the mechanism of the monolayer formation of Bergmann glia is poorly understood. Several reports have suggested that Notch signaling plays instructive roles in promoting the identities of several types of glial cells, including Bergmann glia. Moreover. Notch receptors are expressed in Bergmann glia during development. Here, we have deleted the Notch 1, Notch2 and RBP-J genes in the Bergmann glia by GFAP-driven, Cre-mediated recombination, to study the role of Notch-RBP-J-signaling in the monolayer formation of Bergmann glia. Notch 1/2- and RBP-J-conditional mutant mice showed disorganization of Bergmann fibers, irregularities of the Bergmann glial lining and aberrant localization of Bergmann glia in the molecular layer. Thus, Notch-RBP-J signaling plays crucial roles in the monolayer formation and morphogenesis of Bergmann glia. (C) 2007 Elsevier Inc. All rights reserved.