CEREBRAL METABOLITE DYNAMICS DURING TEMPORARY COMPLETE ISCHEMIA IN RATS MONITORED BY TIME-SHARED H-1 AND P-31 NMR-SPECTROSCOPY
CEREBRAL METABOLITE DYNAMICS DURING TEMPORARY COMPLETE ISCHEMIA IN RATS MONITORED BY TIME-SHARED H-1 AND P-31 NMR-SPECTROSCOPY
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DOI：
10.1002/mrm.1910130103
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发表时间：
1990-01-01
影响因子：
3.3
通讯作者：
JAMES, TL
中科院分区：
文献类型：
--
作者：
CHANG, LH;SHIRANE, R;JAMES, TL
采用分时1H和31P体外核磁共振波谱法监测大鼠全脑缺血30min和再灌注2h时脑磷代谢物、细胞内pH和乳酸的变化。缺血诱导后，细胞内pH值从7.14 +-下降。0.01 ~ 6.32 .+-。0.10，乳酸浓度从1.6 +-升高。0.4至15.8 .+-。2.5 .mu.mol / g;ATP和磷酸肌酸完全耗尽，而无机磷酸盐增加715 +-。47%。血流恢复后1小时内，高能磷酸盐和乳酸水平恢复到接近基线水平。缺血再灌注时细胞内pH和乳酸水平的变化具有良好的相关性。
The changes in cerebral phosphorus metabolites, intracellular pH, and lactate during 30 min of complete global ischemia and 2 h of reperfusion were monitored by time-shared 1H and 31P in vitro NMR spectroscopy in rats. After the induction of ischemia, intracellular pH decreased from 7.14 .+-. 0.01 to 6.32 .+-. 0.10, and lactate concentration increased from 1.6 .+-. 0.4 to 15.8 .+-. 2.5 .mu.mol/g; ATP and phosphocreatine were totally depleted, while inorganic phosphate increased 715 .+-. 47%. Within 1 h after blood flow was restored, high-energy phosphates and lactate levels had recovered close to baseline levels. The changes in intracellular pH and lactate levels during ischemia and reperfusion correlated well.