EFFECT OF DIFFERENTIATION ON REPAIR OF DNA SINGLE-STRAND BREAKS IN NEUROBLASTOMA-CELLS
EFFECT OF DIFFERENTIATION ON REPAIR OF DNA SINGLE-STRAND BREAKS IN NEUROBLASTOMA-CELLS
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DOI：
10.1016/s0006-291x(75)80444-x
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发表时间：
1975-01-01
影响因子：
3.1
通讯作者：
FINKLESTEIN, JZ
中科院分区：
文献类型：
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作者：
BYFIELD, JE;LEE, YC;FINKLESTEIN, JZ
小鼠C-1300神经母细胞瘤细胞修复X射线诱导的DNA单链断裂的能力进行了比较，在两个不同的培养条件下。在含有10%胎牛血清的正常培养基中生长的未分化细胞在30分钟的修复期内显示出实质性的断裂重新连接。无血清培养基中的非生长分化细胞似乎不能修复这种断裂。这种现象是否归因于修复酶的损失或与不利培养条件相关的分化细胞的不稳定性增加尚不确定。结果表明：（a）核完整性对C-1300神经母细胞的形态发生分化并不是绝对重要的，（B）也意味着组织培养生长条件的恶化可能以独立于其他复杂生物合成事件的方式影响DNA修复机制。
The capacity of murine C-1300 neuroblastoma cells to repair x-ray induced DNA single strand breaks was compared under two distinct culture conditions. Growing undifferentiated cells maintained in normal medium containing 10% fetal calf serum showed substantial break rejoining during a 30 minute repair period. Non-growing, differentiated cells in serum-free medium did not appear to be capable of repairing such breaks. Whether this phenomenon is attributable to a loss of repair enzymes or to an increased lability of the differentiated cells related to adverse culture conditions is uncertain. The results suggest that (a) nuclear integrity is not absolutely vital to morphogenetic differentiation of C-1300 neuroblasts and (b) also imply that deterioration of tissue culture growth conditions may affect DNA repair mechanisms in a fashion independent of other complex biosynthetic events.