Confirmation of Berberis spp.as alternate hosts of Puccinia striiformis f.sp.tritici on wheat in China
Confirmation of Berberis spp.as alternate hosts of Puccinia striiformis f.sp.tritici on wheat in China
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发表时间：
2011
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通讯作者：
Zhao Jie
中科院分区：
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作者：
Zhao Jie
pypynia和aecia产自Berberis shensiana，B。利用小麦条锈菌的萌发孢子接种陕西地区的条锈菌和多分枝芽孢杆菌，用接种的条锈菌产生的芽孢接种小麦品种名县169，在小麦叶片上产生典型的条锈菌。聚合酶链反应和DNA序列分析证实，该锈病菌为小麦条锈病菌p.s rififormis f.sp.tritrii。利用田间收集的小麦条锈病叶片的芽孢杆菌接种小麦名县169，产生典型的条锈病菌。因此，在自然条件下，小檗（Berberis spp.）不仅是中国小麦条锈病的代用寄主，而且是小麦条锈病的代用寄主。striifromis f.sp。这一发现将为小麦条锈病高水平的遗传多样性和毒力变异提供更令人满意的解释，并为今后我国小麦条锈病防治研究提供有力的支持。
Pycnia and aecia were produced on Berberis shensiana,B.potaninii and B.dolichobotrys in Shaanxi Province after inoculation using germinating telia of Puccinia striiformis f.sp.tritici.Wheat cultivar Mingxian 169 was inoculated with aeciospores produced from the inoculated B.shensiana,and resulted in typical uredia of P.striiformis on the wheat leaves.Analyses using polymerase chain reaction and DNA sequence confirmed the rust fungus as P.striiformis f.sp.tritici.Wheat Mingxian 169 inoculated using aeciospores from the rust-infected leaves of B.shensiana collected from fields,produced uredia typical of stripe rust.In natural conditions,therefore,it demonstrated barberries(Berberis spp.) not only serve as alternate hosts for stripe rust pathogen of wheat in China,but the pathogen of wheat stripe rust,P.striifromis f.sp.tritici,can also perform its sexual stage on Berberis spp.This finding will provide a more satisfactory interpretation for high level of genetic diversity and virulence variation,and a powerful support to improve strategies for a better control of wheat stripe rust in future studies in the country.