Effect of prenatal PFOS exposure on liver cell function in neonatal mice
Effect of prenatal PFOS exposure on liver cell function in neonatal mice
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产前PFOS暴露对新生小鼠肝细胞功能的影响
DOI：
10.1007/s11356-019-05245-4
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发表时间：
2019-06-01
影响因子：
5.8
通讯作者：
Yang, Bin
中科院分区：
文献类型：
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作者：
Liang, Xiaoliu;Xie, Guojie;Yang, Bin
全氟辛烷磺酸（PFOS）是一种肝毒性污染物，在人类脐带血中被检测到，它可能对胚胎造成健康风险。本研究通过小鼠宫内全氟辛烷磺酸暴露实验，通过生化试验、定量PCR和免疫染色等方法，评估全氟辛烷磺酸对产后1天（PND1）子代的潜在影响。结果显示，在高剂量5 mg /kg的PFOS剂量下，暴露于PFOS的母鼠肝脏明显变大，并诱发肝脂肪变性。PND1小鼠肝内甘油三酯、总胆固醇和低密度脂蛋白含量因高剂量PFOS暴露而升高，而细胞内HDL含量降低。定量PCR显示，与不含pfos的对照组相比，PND1肝脏中负责脂肪酸氧化的细胞色素P4A14 （CYP4A14）、负责肝脏脂肪酸摄取的CD36、以及负责肝脏脂质输出的载脂蛋白B100 （APOB）和成纤维细胞生长因子21 （FGF21）的功能信使rna发生了变化。在进一步的验证中，免疫荧光染色显示PND1小鼠暴露于pfos后，肝脏CYP4A14和CD36免疫反应细胞增加。此外，PND1肝脏中APOB和FGF21免疫荧光阳性细胞减少。总之，这些初步发现表明，产前暴露于全氟辛烷磺酸可能会影响PND1小鼠肝细胞的脂质代谢。
Perfluorooctane sulfonate (PFOS), a hepatotoxic pollutant, is detected in the human cord blood, and it may induce health risk to an embryo. In this study, we established intrauterine exposure to PFOS in mice to evaluate potential impacts of PFOS on postnatal day 1 (PND1) offspring through conducting biochemical tests, quantitative PCR, and immunostaining. As results, PFOS-exposed maternal mice showed marked hepatomegaly and induced liver steatosis in a high dose of 5 mg PFOS/kg. In PND1 mice, intrahepatic contents of triglyceride, total cholesterol, and LDL were elevated by high-dose PFOS exposure, while intracellular HDL content was decreased. As shown in quantitative PCR, functional messenger RNAs of cytochrome P4A14 (CYP4A14) for fatty acid oxidation, CD36 for hepatic fatty acid uptake, and apolipoprotein B100 (APOB) and fibroblast growth factor 21 (FGF21) for hepatic export of lipids in PND1 livers were changed when compared to those in PFOS-free controls. In further validations, immunofluorescence stains showed that hepatic CYP4A14 and CD36 immunoreactive cells were increased in PFOS-exposed PND1 mice. In addition, reduced immunofluorescence-positive cells of APOB and FGF21 were observed in PND1 livers. Collectively, these preliminary findings demonstrate that prenatal exposure to PFOS may affect lipid metabolism in liver cells of PND1 mice.