Mediation by nitric oxide of the stimulatory effects of ethanol on blood flow
Mediation by nitric oxide of the stimulatory effects of ethanol on blood flow
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DOI：
10.1016/s0024-3205(02)01572-2
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发表时间：
2002-05-10
期刊：
影响因子：
6.1
通讯作者：
Lieber, CS
中科院分区：
文献类型：
--
作者：
Baraona, E;Shoichet, L;Lieber, CS
在通过胃管给予乙醇或水的大鼠中评估一氧化氮（NO）对与酒精氧化相关的血液动力学效应的贡献，所述大鼠有或没有用NO合酶抑制剂N-ω-单甲基-L-精氨酸（L-NMMA; 15 mg/Kg i. p.）或醇脱氢酶抑制剂4-甲基吡唑（4-MP; 82 mg/Kg i. p.）。酒精使血浆NO（用化学发光法测定）增加63%。这是由L-NMMA或4-MP预防的。心输出量和局部血流量用（57）钴标记微球测定。酒精显著增加门静脉血流量（130 +/- 6 mL/min/Kg对62 +/- 4，对照组; p < 0.01），肝、脾或胰腺动脉血流量无变化，表明血管舒张主要是肠系膜。此外，它使冠状动脉血流量增加了四倍，使肾脏血流量增加了一倍，心输出量增加了38%，而肺、脑或睾丸血流量没有显著变化。所有的刺激作用，乙醇对流量，以及NO水平的上升，防止由L-NMNIA，定罪NO作为介体的血液动力学效应的乙醇氧化，可能通过乙酸和腺苷的作用。(C)2002年爱思唯尔科学公司所有的灯都保留。
The contribution of nitric oxide (NO) to the hemodynamic effects associated with alcohol oxidation was assessed in rats given either ethanol or water by gastric tube, with and without pre-treatment with either the NO synthase inhibitor N-omega-monomethyl-L-arginine (L-NMMA; 15 mg/Kg i.p.) or the alcohol dehydrogenase inhibitor 4-methylpyrazole (4-MP; 82 mg/Kg i.p.). Alcohol increased plasma NO (measured with chemiluminescence) by 63%. This was prevented by either L-NMMA or 4-MP. Cardiac output and regional blood flows were determined with (57)Cobalt-labeled microspheres. Alcohol markedly enhanced portal blood flow (130 +/- 6 mL/min/Kg vs. 62 +/- 4, in controls; p < 0.01) with no changes in the hepatic, splenic or pancreatic arterial blood flows, indicating that the vasodilatation is mainly mesenteric. In addition, it quadrupled the coronary blood flow, doubled the renal flow and increased cardiac output by 38%, with no significant changes in pulmonary, cerebral or testicular flows. All the stimulatory effects of ethanol on flow, as well as the rise in NO levels, were prevented by L-NMNIA, incriminating NO as the mediator of the hemodynamic effects of ethanol oxidation, acting probably via acetate and adenosine. (C) 2002 Elsevier Science Inc. All lights reserved.