Purification of Lactobacillus acidophilus surface-layer protein and its immunomodulatory effects on RAW264.7 cells
Purification of Lactobacillus acidophilus surface-layer protein and its immunomodulatory effects on RAW264.7 cells
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嗜酸乳杆菌表面层蛋白的纯化及其对RAW264.7细胞的免疫调节作用
DOI：
10.1002/jsfa.8294
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发表时间：
2017-09-01
影响因子：
4.1
通讯作者：
Pan, Daodong
中科院分区：
文献类型：
--
作者：
Zhang, Dandan;Wu, Mengting;Pan, Daodong
BACKGROUNDSurface-layer proteins (SLP) have been found in the outermost layer of the cell wall in many types of lactobacillus are considered to be an important factor with respect to intestinal immunity.RESULTSThe present study compared the effects of SLP extracted by different concentrations of LiCl and carbamide, and subsequently identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, circular dichroism and differential scanning calorimetry. Furthermore, RAW 264.7 cells were used to evaluate the immunomodulatory effects of SLP. SLP were derived from Lactobacillus acidophilusCICC6074 with a molecular weight of 46kDa, and consisted of 16.9% -helix, 42.3% -sheet, 20.8% -turns and 22.5% random coils. SLP promoted NO secretion and higher quantities of NO were produced as the SLP concentrations increased. SLP concentrations over 50 mu gmL(-1) significantly decreased the amount of tumor necrosis factor- secreted by RAW264.7 cells.CONCLUSIONSLP can trigger immunomodulatory effects in RAW 264.7 cells. This provides crucial information that will enable the further use of L.acidophilus in food, medicine and other products. (c) 2017 Society of Chemical Industry
