Microchip-based enzyme-linked immunosorbent assay (microELISA) system with thermal lens detection
Microchip-based enzyme-linked immunosorbent assay (microELISA) system with thermal lens detection
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DOI：
10.1039/b411356j
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发表时间：
2004-01-01
期刊：
影响因子：
6.1
通讯作者：
Kitamori, T
中科院分区：
文献类型：
--
作者：
Sato, K;Yamanaka, M;Kitamori, T
建立了一种基于微芯片的酶联免疫吸附分析系统，并成功地测定了干扰素-γ。该系统由具有Y形微通道和堤坝结构的微芯片、聚苯乙烯微珠和热透镜显微镜(TLM)组成。免疫分析所需的所有反应都是通过连续引入样品和试剂在微通道中进行的。使用TLM作为底物溶液，在通道的下游检测到液相中的酶反应产物。通过芯片集成，缩短了抗原抗体反应时间。采用酶标记法，提高了测定的下限。此外，检测程序大大简化，检测所需时间显著缩短。该系统具有作为小型化、自动化的高通量分析仪的良好发展潜力。
A microchip-based enzyme-linked immunosorbent assay (microELISA) system was developed and interferon-gamma was successfully determined. The system was composed of a microchip with a Y-shaped microchannel and a dam structure, polystyrene microbeads, and a thermal lens microscope (TLM). All reactions required for the immunoassay were done in the microchannel by successive introduction of a sample and regents. The enzyme reaction product, in a liquid phase, was detected downstream in the channel using the TLM as substrate solution was injected. The antigen-antibody reaction time was shortened by the microchip integration. The limit of the determination was improved by adopting the enzyme label. Moreover, detection procedures were greatly simplified and required time for the detection was significantly cut. The system has good potential to be developed as a small and automated high throughput analyzer.