Rat brain expresses an alternatively spliced form of the dihydropyridine-sensitive L-type calcium channel alpha 2 subunit.
Rat brain expresses an alternatively spliced form of the dihydropyridine-sensitive L-type calcium channel alpha 2 subunit.
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大鼠大脑表达二氢吡啶敏感的 L 型钙通道 α2 亚基的选择性剪接形式。
DOI：
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发表时间：
1992
影响因子：
11.1
通讯作者：
H. Chin
中科院分区：
文献类型：
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作者：
H. Kim;H. Kim;P. Lee;R. G. King;H. Chin
我们克隆并测序了编码大鼠脑电压敏感的L型钙通道（二氢吡啶受体）的α2亚基的cDNA（rB-α2）。该cDNA（3823个碱基对）编码1091个氨基酸的蛋白质，Mr为123,822。 rB-α2 cDNA 推导的氨基酸序列与兔骨骼肌α2 亚基的氨基酸序列高度相似（95% 氨基酸同一性）。 rB-α 2 蛋白不同于先前克隆的骨骼肌 α 2 亚基蛋白 [Ellis, S. B.、Williams, M. E.、Ways, N. R.、Brenner, R.、Sharp, A. H.、Leung, A. T.、Campbell, K. P.、McKenna, E.、Koch, W.、Hui, A.、Schwartz, A. & Harpold, M. M. (1988) Science 241, 1661-1664]因为它在假定的跨膜结构域 1 和 2 之间包含 7 个氨基酸残基的插入和 19 个氨基酸片段的删除。我们表明 rB-α 2 和骨骼肌 α 2 亚基转录物是初级转录物选择性剪接产生的变体，并且它们在大脑和骨骼肌中差异表达，分别。
We have cloned and sequenced a cDNA (rB-alpha 2) encoding an alpha 2 subunit of the voltage-sensitive L-type calcium channel (dihydropyridine receptor) of rat brain. The cDNA (3823 base pairs) encodes a protein of 1091 amino acids with a Mr of 123,822. The deduced amino acid sequence of rB-alpha 2 cDNA is highly similar (95% amino acid identity) to that of rabbit skeletal muscle alpha 2 subunit. The rB-alpha 2 protein is distinct from the previously cloned skeletal muscle alpha 2-subunit protein [Ellis, S. B., Williams, M. E., Ways, N. R., Brenner, R., Sharp, A. H., Leung, A. T., Campbell, K. P., McKenna, E., Koch, W., Hui, A., Schwartz, A. & Harpold, M. M. (1988) Science 241, 1661-1664] because it contains an insertion of 7 amino acid residues and a deletion of a 19-amino acid segment between putative transmembrane domains 1 and 2. We show that the rB-alpha 2 and skeletal muscle alpha 2-subunit transcripts are the variants produced by alternative splicing of the primary transcript and that they are differentially expressed in brain and skeletal muscle, respectively.