Imaging cortical vasculature with stimulated Raman scattering and two-photon photothermal lensing microscopy
Imaging cortical vasculature with stimulated Raman scattering and two-photon photothermal lensing microscopy
复制标题
DOI：
10.1002/jrs.3156
复制
发表时间：
2012-05-01
影响因子：
2.5
通讯作者：
Uchegbu, Ijeoma
中科院分区：
文献类型：
--
作者：
Moger, Julian;Garrett, Natalie. L.;Uchegbu, Ijeoma
绘制微血管形态和血液动力学参数（如血容量）的能力对于许多生物医学研究是理想的，并将导致对血管生成和血管疾病机制的更深入理解。毛细血管网络可以描绘在三维与双光子激发荧光显微镜，然而，这需要静脉注射的荧光染料到血浆中，这往往复杂化在体内成像，并只提供了一个间接的估计局部红细胞压积体积。此外，可视化毛细血管的空间分布通常是不够的;理想情况下，人们希望将血管的接近度与周围的局部组织结构相关联。在这项研究中，我们提出了一种新的多模式的方法，结合受激拉曼散射和双光子光热透镜，以提供同时可视化的皮质微血管形态和周围的细胞结构。我们表明，体积分析的非线性光热对比的红细胞允许直接量化的本地红细胞压积的体积，而不是依赖于平均血浆体积-红细胞压积比。版权所有（c）2012约翰威利父子有限公司
The ability to map microvascular morphology and hemodynamic parameters, such as blood volume, is desirable for many biomedical studies and will lead to a deeper understanding of the mechanisms of angiogenesis and vascular disease. Capillary networks can be delineated in three dimensions with two-photon excited fluorescence microscopy; however, this requires the intravenous infusion of a fluorescent dye into the blood plasma, which often complicates in vivo imaging and only provides an indirect estimate of local haematocrit volume. Moreover, visualising the spatial distribution of capillaries is often insufficient; ideally, one would wish to correlate the proximity of the blood vessels with the surrounding local tissue structure. We present in this study a novel multimodal approach that combines stimulated Raman scattering and two-photon photothermal lensing to provide simultaneous visualisation of cortical microvasular morphology and surrounding cellular structures. We show that volumetric analysis of the nonlinear photothermal contrast of erythrocytes allows a direct quantification of local haematocrit volume rather than relying upon average plasma volume-to-haematocrit ratios. Copyright (c) 2012 John Wiley & Sons, Ltd.