Expression of the M-CSF (CSF-1) gene by human monocytes.
Expression of the M-CSF (CSF-1) gene by human monocytes.
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DOI：
10.1182/blood.v69.5.1409.1409
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发表时间：
1987-05
期刊：
影响因子：
20.3
通讯作者：
A. Rambaldi;D. Young;James D. Griffin
中科院分区：
文献类型：
--
作者：
A. Rambaldi;D. Young;James D. Griffin
单核细胞集落刺激因子（M-CSF、CSF-1）是一种巨噬细胞谱系特异性生长因子。使用人 M-CSF cDNA 探针的 Northern 印迹分析和人 M-CSF 的特异性生物测定法用于研究 M-CSF 的细胞来源。在佛波醇肉豆蔻酸酯乙酸酯 (PMA) 或 γ-干扰素刺激的血液单核细胞中诱导 M-CSF 基因的表达。当单核细胞被分离成高度纯化的 T 细胞和单核细胞群时，主要在单核细胞中检测到 M-CSF 转录物。此外，用 PMA 刺激的单核细胞释放出类似 M-CSF 的活性。这些结果证明M-CSF可以由巨噬细胞谱系的活化细胞产生。
Monocyte colony-stimulating factor (M-CSF, CSF-1) is a macrophage lineage-specific growth factor. Northern blot analysis using a human M-CSF cDNA probe and a specific bioassay for human M-CSF were used to investigate the cellular sources of M-CSF. Expression of the M-CSF gene was induced in blood mononuclear cells stimulated by phorbol myristate acetate (PMA) or gamma-interferon. When mononuclear cells were fractionated into highly purified populations of T cells and monocytes, M-CSF transcripts were detected predominantly in monocytes. Further, monocytes stimulated with PMA released an M-CSF-like activity. These results demonstrate that M-CSF can be produced by activated cells of the macrophage lineage.