Intravenous Injections in Neonatal Mice
Intravenous Injections in Neonatal Mice
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DOI：
10.3791/52037
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发表时间：
2014-11-01
影响因子：
1.2
通讯作者：
Foust, Kevin D.
中科院分区：
文献类型：
--
作者：
Lampe, Sara E. Gombash;Kaspar, Brian K.;Foust, Kevin D.
静脉注射是一种临床适用的递送治疗的方式。对于成年啮齿动物和较大的动物，静脉注射在技术上是可行的并且是常规的。然而，一些小鼠模型可能会出现疾病早期发作且进展迅速的情况，这使得潜在疗法的实施变得困难。颞（或面部）静脉位于小鼠耳芽的前面，在出生后的头两天使用解剖显微镜可以在头部的两侧清晰可见。在此窗口期间，颞静脉可注射最多 50 μl 的体积。注射是安全的，幼犬和母犬都能很好地耐受。典型的注射程序在 1-2 分钟内完成，然后将幼犬放回笼子。到出生后第三天，静脉很难显现，注射过程在技术上变得不可靠。该技术已用于递送腺相关病毒（AAV）载体，根据所选的病毒血清型，该载体可以在动物的一生中提供几乎全身稳定的转基因表达。
Intravenous injection is a clinically applicable manner to deliver therapeutics. For adult rodents and larger animals, intravenous injections are technically feasible and routine. However, some mouse models can have early onset of disease with a rapid progression that makes administration of potential therapies difficult. The temporal (or facial) vein is just anterior to the ear bud in mice and is clearly visible for the first two days after birth on either side of the head using a dissecting microscope. During this window, the temporal vein can be injected with volumes up to 50 mu l. The injection is safe and well tolerated by both the pups and the dams. A typical injection procedure is completed within 1-2 min, after which the pup is returned to the home cage. By the third postnatal day the vein is difficult to visualize and the injection procedure becomes technically unreliable. This technique has been used for delivery of adeno-associated virus (AAV) vectors, which in turn can provide almost body-wide, stable transgene expression for the life of the animal depending on the viral serotype chosen.